Toyo Jozo Company, Ltd.

Asahi Kasei Kogyo Kabushiki Kaisha

Hitoshi Sagai

Harumi Masujima

Mamoru Takahashi

Shigeo Katsuragi

Masayasu Takahara

Junboku Kajiwara

Shigeru Ikuta

Koji Suzuki

Keiko Nogata

Kimiko Hattori

A polypeptide represented by the following general formula (I): ##STR1## wherein X.sub.1 means a hydrogen atom, acetyl group of amino acid residue, X.sub.2 and X.sub.3 are either the same or different and mean individually an amino acid residue, and when X.sub.2 stands for Cys, X.sub.3 denotes an amino acid residue other than Cys.

DNA encoding human CV/2n superoxide dismutase muteins with at least

Glucose-6-phosphate dehydrogenase which is an enzyme for assaying creatinekinase for diagnolyzation of myocardial infraction is produced by culturing a transformant having DNA which codes amino acid sequence shown from its N-terminal in FIG. 1 and which is extraneous to a host to have genetic information of the DNA expressed and collecting glucose-6-phosphate dehydrogenase from the culture.

Gene and process of making glucose-6-phosphate dehydrogenase

The disclosure is made on a polydeoxyribonucleic acid having a base sequence encoding an amino acid sequence of a polypeptide which is a creatinase derived from a creatinase-producing microorganism belonging to the genus of Bacillus. The bsae sequence of the creatinase gene DNA and amino acid sequence of the creatinase have been clarified. The transformant having this polydeoxyribonucleic acid as well as a process for preparing the creatinase at a high productivity with the application of genetic engineering technique are disclosed.

DNA encoding a bacillus creatinase

DNA having a base sequence encoding a specified amino acid sequence of a polypeptide constituting an L-.alpha.-glycerophosphate oxidase (GPO) is disclosed. A polypeptide having the specified amino acid sequence can be produced by inserting the DNA into a plasmid, culturing a transformant produced by introducing this plasmid into a host microorganism which is selected from microorganisms belonging to Escherichia coli, Bacillus subtilis, or Saccharomyces cerevisiae. The GPO is useful for the quantitative analysis of triglyceride, glycerine, etc., and the determination of lipase activity, when used in combination with other enzymes such as lipase, glycerol kinase, or the like, or with an ATP reagent.

DNA having genetic information of L-.alpha.-glycerophosphate oxidase and

A polypeptide represented by the following general formula (I):

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Mishima, Japan

Hitoshi Sagai