Laboratory of Molecular Biophotonics

Hitoshi Nohta

Masatoshi Yamaguchi

Hideyuki Yoshida

Hirofumi Shiono

Tomomi Suzuki

Shigetoshi Okazaki

Hiroko Iida

Kivoshi Zaitsu

Chika Utsuyama

Junichi Ishida

The method of analyzing polyphenols and bisphenols in accordance with the present invention comprises a step of reacting a bisphenol and/or a polyphenol with a labeling reagent having a pyrene group, so as to generate a fluorescent derivative; a step of irradiating the fluorescent derivative with excitation light and detecting fluorescence emitted from the fluorescent derivative; and a step of calculating an amount of the bisphenol and/or polyphenol in a test sample according to an intensity of thus detected fluorescence and a relationship between a known concentration of a bisphenol and/or a polyphenol contained in a standard sample and fluorescence intensity.

Method of analyzing bisphenols and polyphenols

This invention provides rotaxane type dyes. Specifically, the rotaxane type dye according to the invention has a rotaxane structure which binds dye molecules to both of the termini of a chain group penetrating a cyclodextrin ring, which possesses excellent water-solubility, and which is capable of having a plurality of different dyes.

Cyclodextrin-based rotaxane dyes, labeling agent using the dye, and a method for labeling

The enzyme substrate according to this invention has within its molecule both a group to be cleaved by an enzyme reaction and a group that forms a strongly fluorescent coumarin derivative through intramolecular lactonization when cleaved by the enzyme reaction. Furthermore, the method for determining an enzyme activity according to this invention comprises conducting the enzyme reaction by the use of the enzyme substrate of the invention and determining the enzyme activity by means of the measurement of fluorescence of the coumarin derivative formed.

Fluorescent enzyme substrates and method for determining enzyme activities

A method and a device for the analysis of 5-hydroxyindoles or catecholamines with high sensitivity. New chemiluminescence labeling agents, 6-aminomethylphthalhydrazide or 1,2-bis(phthalhydrazino)ethylenediamine, are reacted with 5-hydroxyindoles or catecholamines to form their stable derivatives. The derivatives emit strong luminescence in the presence of an oxidizing agent. In the chemiluminescence detection method, there is extremely low background noise and thus the method enables analysis with high sensitivity. The analysis of 5-hydroxyindoles or catecholamines in a sample is enabled by a method (1), in which each of the above-mentioned components is separated, reacted with said chemiluminescence labeling agent to form its derivative, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected, or by another method (2), in which the mixture of 5-hydroxyindoles or catecholamines in a sample is reacted with the chemiluminescence labeling agent to form its derivative mixture, subsequently each derivative is separated, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected.

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Fukuoka, Japan

Hitoshi Nohta