The University of Tokyo

Kyushu University

Waseda University

Hitoshi Kurumizaka

Hiroshi Kimura

Yuko Sato

Shinji Takeoka

Motomu Kanai

Hiroki Suto

Isao Sakane

Yosuke Obata

Kenzo Yamatsugu

Yoko Hayashi

Nozomu Nagashima

Syunsuke Saito

Namiko Ikegaya

Naoya Takeda

Yasuhiro Arimura

Akihisa Osakabe

Yasuyuki Ohkawa

Yuki Aoi

Yoshifumi Amamoto

Shigehiro Kawashima

Tetsuya Handa

Akihito Harada

A method is provided for integrating a DNA fragment of a desired base sequence into a site located adjacent to a binding region of a DNA-binding protein bound to a DNA molecule, the method including bringing the DNA fragment having a base sequence including a transposase-binding sequence and the desired base sequence close to the binding region using a specific binding substance to the DNA-binding protein, binding transposase to the transposase-binding sequence, and activating the transposase such that the DNA fragment of the desired base sequence is integrated into the site located adjacent to the binding region.

Method for inserting desired DNA fragment into site located adjacent to binding domain of DNA-binding protein

An artificial catalyst system which can acylate chromosome proteins with high selectivity has successfully been established by using a combination of an acyl CoA activating catalyst having target acylation area binding ability and acyl CoA or a derivative thereof.

Artificial catalyst system for selective acylation of chromosome protein

The present invention provides a reagent for introducing a protein or gene into a cell. The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (wherein in formula (I)-1: L is a single bond, &#x2014;CONH&#x2014;, or &#x2014;S&#x2014;S&#x2014;; Mis &#x2014;(CH)&#x2014; or &#x2014;(CHCHO)&#x2014; (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).

Reagent for introduction of protein or gene

Growing community of inventors

Tokyo, Japan

Hitoshi Kurumizaka