Nippon Shoji Kabushiki Kaisha

Hitoshi Kaneta

Hiroki Tanaka

Yasuji Soda

Chozo Hayashi

Mieko Fujio

Shigeki Ito

Yoji Marui

Toshihide Nagasaki

An enzyme immunoassay for an antigen using a solid phase, which employs (a) a biotinylated antibody in which a biotin derivative is bound to a thiol group of an Fab' fragment of an antibody, (b) an enzyme-labelled antibody, and (c) a solid phase immobilized with a substance capable of specifically reacting with the above biotin derivative selected from the group consisting of avidin, streptoavidin and a derivative thereof which is bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, or alternatively employs (a') a solid phase immobilized with a biotinylated antibody via a substance selected from the group consisting of avidin, streptoavidin and a derivative thereof wherein said substance is bound to the biotin moiety of said biotinylated antibody and is also bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, and (b') an enzyme-labelled antibody, and a solid phase used therefor.

Enzyme immunoassay for antigen and solid phase used therefor

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Takatsuki, Japan

Hitoshi Kaneta