Kyushu University

Nippon Suisan Kaisha, Ltd.

University of Miyazaki

Konan Gakuen

Hitoshi Izumida

Shinichi Sugimoto

Makoto Ito

Yuji Okita

Daisuke Honda

Naoki Nagano

Masahiro Hayashi

Takanori Matsuda

Keishi Sakaguchi

Yosuke Taoka

Takumi Kobayashi

A microbial oil is extracted from stramenopile transformed with a gene associated with synthesis of fatty acids, the gene encoding a fatty acid desaturase. The stramenopile belongs to the class Labyrinthulomycete. The microbial oil satisfies one or more of the following requirements: (a) an amount of arachidonic acid is 7% or less based on a total amount of the fatty acid composition; (b) an amount of DPA is 9% or less based on the total amount of the fatty acid composition; (c) an amount of ETA is 0.04% or more based on the total amount of the fatty acid composition; (d) an amount of EPA is 7% or more based on the total amount of the fatty acid composition; and (e) an amount of DHA is 45% or more based on the total amount of the fatty acid composition.

Microbial oil extracted from stramenopiles

Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class, more specifically, to a genus, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (&#x394;5 desaturase gene, &#x394;12 desaturase gene and/or &#x3c9;3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

Method for transforming stramenopiles

Growing community of inventors

Tokyo, Japan

Hitoshi Izumida