Ajinomoto Co., Ltd.

Hitoshi Enei

Osamu Kurahashi

Takayasu Tsuchida

Katsuaki Sato

Yoshio Hirose

Hiroki Kawashima

Shigeru Nakamori

Hisao Ito

Kazuhiko Matsui

Konosuke Sano

Haruo Uchibori

Mitsuyoshi Seki

Masaru Ishihara

Koichi Takinami

Takashi Tanaka

Ryosuke Uchio

Kenji Kikuchi

Shoji Sakurai

Eiji Goto

Masahiro Kamada

Yasuo Anzai

Morikatsu Ishii

A process for the production of L-glutamic acid comprising growing microorganisms belonging to the genera Brevibacterium and Corynebacterium that are resisted to prumycin.

Process for producing L-glutamic acid

The present invention relates to a process for producing an aromatic amino acid which comprises culturing a Coryneform bacterium carrying a recombinant DNA constructed by connecting (1) a gene capable of being expressed in a Coryneform bacterial cell and coding for 3-deoxy-D-arabino-heptulonic acid-7-phosphate synthetase with (2) a plasmid vector capable of propagating in a Coryneform bacterial cell.

Coryneform bacteria carrying recombinant DNA and a process for producing

A method for producing L-phenylalanine by fermentation which comprises aerobically culturing an L-phenylalanine-producing microorganism in an aqueous culture medium and recovering the L-phenylalanine accumulated in the culture medium, the L-phenylalanine-producing microorganism having been constructed by incorporating a recombinant plasmid DNA, into which a DNA fragment controlling resistance to a phenylalanine antagoinst, obtained from a chromosomal DNA of a mutant of the genus Bacillus resistant to the phenylalanine antagonist, has been inserted, into a recipient strain of the genus Bacillus.

Method for producing L-phenylalanine by fermentation

A method for producing L-tryptophan by fermentation in high yields employing a microorganism of the genus Bacillus, which has been constructed by a gene splicing technique by incorporating a recombinant plasmid DNA containing an inserted DNA containing fragment controlling resistance to tryptophan-antagonists into a recipient strain of the genus Bacillus. The incorporated DNA fragment is a mutant of the genus Bacillus which was obtained from resistant to tryptophan-antagonists.

Method for producing L-tryptophan by fermentation

A process for economically producing L-tryptophan which involves culturing aerobically in a culture medium a mutant of the genus Bacillus which is resistant to azaserine and a tryptophan analogue and recovering the L-tryptophan produced which accumulates in the culture medium.

Process for producing L-tryptophan by fermentation

L-Histidine producing microorganisms which have been constructed by introducing a recombinant plasmid DNA inserted on a chromosomal DNA fragment into a recipient strain of the genus Bacillus. These microorganisms are employed to produce L-histidine in higher than normal yields. The recombinant plasmid DNA inserted is obtained from a donor mutant strain of Bacillus subtilis which is resistant to certain L-histidine antagonists. The resistant plasmid confers the properties of the mutant strain upon the recipient Bacillus subtilis to make its high yield of product even higher.

Method for producing L-histidine by fermentation

When sensitivity to decoyinine is given by mutation to L-phenylalanine-producing mutants of the genus Brevibacterium and Corynebacterium, the productivity of L-phenylalanine of the new mutants is improved.

Improved method for producing pyruvic acid by fermentation of pyruvic acid producing mutants of the strain Candida lipolytica which require methionine and thiamine for growth.

Process for producing pyruvic acid by fermentation

Method for producing guanosine by culturing a guanosine-producing mutant of the genus Bacillus which requires adenine for growth, and is resistant to at least one sulfa drug.

Method of producing guanosine by fermentation

Mutant strains of Bacillus subtilis which require adenine for growth and are resistant to at least one sulfa drug produce inosine in culture medium.

Method for producing inosine by fermentation

Production of guanosine by fermentation with a Bacillus mutant requiring adenine for growth and resistant to psicofuranine or decoyinine.

Growing community of inventors

Zushi, Japan

Hitoshi Enei