Amano Enzyme Inc.

Amano Pharmaceutical Co., Ltd.

National Research Institute of Brewing

Hitoshi Amano

Shotaro Yamaguchi

Akiko Matsunaga

Masamichi Okada

Yoshiaki Kurono

Naoki Matsumoto

Yuji Nakanishi

Megumi Nakagawa

Kazuo Sato

Akihiro Mizuno

Nobuhiko Mukai

Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.

Stabilized transglutaminase and process for production thereof

Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of: culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.

To provide novel &#x3b2;-amylase derived from a microorganism and a gene thereof. &#x3b2;-amylase derived fromis provided.

Beta-amylase, gene coding therefor and manufacturing method thereof

Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived fromor. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65&#xb0; C. (pH 5.0, 30 min.)

Tannase, gene encoding same, and process for producing same

&#x3b2;-amylase, gene coding therefor and manufacturing method thereof

Fermented malt beverages with reinforced filling taste and fullness of mouthfeel are manufactured by addition of &#x3b1;-glucosidase prior to a heat treatment in a wort production process in the course of manufacturing fermented malt beverages Production of acetic acid can be reduced by addition of &#x3b1;-glucosidase in the fermentation process in the high gravity brewing of beer. Further, low-calorie beers are manufactured by the method in which &#x3b1;-glucosidase is added in the fermentation process in brewing of beer and thereby real fermentation degree is enhanced.

Method of manufacturing fermented malt beverages

The present invention provides a novel ascorbate oxidase (ASOD) which catalyzes oxidation reaction of L-ascorbic acid with molecular oxygen to form L-dehydroascorbic acid and hydrogen peroxide, a process for producing the ascorbate oxidase comprising using a microorganism belonging to the genus Eupenicillium, a gene encoding ASOD, a transformant containing such a gene, a process for producing ASOD using such a transformant, and a reagent composition comprising ASOD, such as a reagent composition for examination, a food additive, and a reagent composition in the fields of food and clinical examination. The ascorbate oxidase has excellent stability particularly in a liquid state.

Growing community of inventors

Kakamigahara, Japan

Hitoshi Amano