Teijin Limited

Tetjin Limited

Hitomi Honda

Kenji Hosoda

Takaharu Kubota

Hideaki Suzuki

Yasuhiko Masuho

Yoshiro Niitsu

Naomi Okamoto

Ryoichi Hasegwwa

Kazuhiko Itou

Kimihiko Matsuzawa

Hiroshi Maruyama

Shigeo Matsuyama

Ichiro Urushizaki

Masayuki Shinmei

A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcinand an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample is provided. A reagent and a kit therefor are also described, as well as a monclonal antibody and a polyclonal antibody used for the assay a process for producing these antibodies; and a process for use of these antibodies.

Method of immunological assaying human osteocalcin, reagent and kit

A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcin and an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. A reagent and a kit therefor are provided. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample, and a reagent and a kit therefor are also provided. A monoclonal antibody and a polyclonal antibody are used for the assay. A process for producing these antibodies, and utilization of these antibodies is described.

Method of immunological assaying of human osteocalcin, reagent and kit

A method of immunologically determining human acid glutathione S-transferase in a human assay sample, which comprises bringing the assay sample into contact with a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of recognizing human acid glutathione S-transferase or an equivalent fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically recognizing human acid glutathione S-transferase or an equivalent fragment of the monoclonal antibody, a method of diagnosing cancer in a human digestive organ by using the above method, a reagent therefor, a kit therefor and a monoclonal antibody for use therein.

Method of determining human acid glutathione S-transferase, reagent,

A method for determining chondrocalcin through an immunoassay using an enzyme(s)-labeled mammalian chondrocalcin or enzyme(s)-labeled anti-chondrocalcin antibody which is characterized by that mammalian body fluid is used as a specimen and immunoreaction in a solution is utilized to determine the chondrocalcin of the mammalian, reagents and a kit therefor. This determination can be utilized for diagnosis or the like for cartilage-relating diseases in mammals.

Method for determining chondrocalcin

An immunoassay method performed in solution wherein a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the same is permitted to exist as the antigen-antibody reaction controller in the immunoreaction solution, and the final concentration of the antigen-antibody controller in the immunoreaction solution is controlled to 0.02 to 0.9% by weight, and a reagent kit to be used for an immunoassay containing the above antigen-antibody reaction controller at a final concentration of 0.02 to 0.9% by weight in the immunoreaction solution, as a part of the constituents thereof.

Immunoassay and reagent kit used therefor

Monoclonal antibody for human acid-glutathione S-transferase and process

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Hino, Japan

Hitomi Honda