Nitta Gelatin Inc.

Kurashiki Boseki Kabushiki Kaisha

Hisayuki Kobayashi

Masahiro Wada

Naoki Inoue

Fumihito Sugihara

Seiko Koizumi

Toshikazu Takano

Kazuhiko Minamigawa

Naohito Kondo

Masahiro Koezuka

Hiroshi Mano

Sachiko Oda

Sachie Nakatani

Hajime Takasaki

Chinfang Liu

Hiroshi Saeki

Masayuki Yasutomi

Keizo Tanisaka

The present invention provide: a novel process for culturing animal cells and a kit for culturing animal cells, in which, even if the number of cells as sampled for biopsy is extremely small, the proliferation can sufficiently be maintained so as to enable to carry out various culture and/or tests, especially anticancer agent sensitivity tests, and the contamination with bacteria can be inhibited without damaging physiological activity of cells, especially sensitivity to anticancer agents. The process for culturing animal cells, according to the present invention, comprises the step of culturing a sample containing animal cells obtained from living body tissue in order to subject the sample to further culture and/or a test, with the process being characterized in that a culture medium is used wherein the culture medium has a proliferating action and physiological activity-retaining action on the animal cells, and further has a killing action and/or multiplication-inhibition action on bacteria.

Kit for culturing cells comprising tube with flat wall, extracellular matrix and culture medium

A problem that the present invention is to solve is to provide: a main body of a peptide molecule which is effective for inhibition of various maladies such as osteoporosis, osteoarthritis and pressure ulcer, particularly, a dipeptide which is easy to absorb into a body in an intestine; a collagen peptide which comprises the dipeptide as an essential dipeptide; and a malady inhibitor which comprises the dipeptide as an essential effective component. As a means of solving such a problem, a collagen peptide according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential dipeptide. A dipeptide according to the present invention is characterized by having a structure of Hyp-Gly. A malady inhibitor according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential effective component.

Collagen peptide, dipeptide and malady inhibitor

Process for culturing cells sampled for biopsy

Method for culturing animal cells in collagen drops on a support

The present invention relates to a method of testing the sensitivity of cancer drugs with cancer cells cultured in vitro. Cancer cells are cultured in a collagen gel substrate. A wide variety of human cancer cell types readily proliferate in the collagen gel substrate, however, fibroblast cells proliferate as well. The measurement of the growth of the cancer cells is hindered by the presence of the fibroblast cells. The present invention solves this problem by counting the number of colonies with an image processor which selectively extracts the image signals of cancer cells and their colonies. In a second embodiment, the growth of cancer cells is determined by measuring the volume of colonies with the image signals of cancer cells and their colonies selectively extracted. The results can be obtained effectively within a short period of time.

Method for testing the sensitivity of anticancer drug

Growing community of inventors

Osaka, Japan

Hisayuki Kobayashi