Ajinomoto Co., Ltd.

Hisashi Yasueda

Shinichi Sugimoto

Yoshiya Gunji

Yoshihiro Usuda

Yousuke Nishio

Yoshio Kawahara

Hiroshi Izui

Akito Chinen

Yoshihiko Hara

Yury Ivanovich Kozlov

Konstantin Vyacheslavovich Rybak

Nobuharu Tsujimoto

Ekaterina Aleksandrovna Slivinskaya

Joanna Yosifovna Katashkina

Masao Motoki

Hisao Ito

Hiroshi Matsui

Seiko Hirano

Ryo Takeshita

Sergei Vladimirovich Mashko

Kazuo Nakanishi

Shuhei Hashiro

Aleksandr Aleksandrovich Krylov

Ippei Inoue

Mayu Mitsuhashi

Yuliya Sergeevna Lobanova

Kazuo Nagase

Kazuhiko Matsui

Nobuo Kato

Keiichi Yokoyama

Yoshimi Kikuchi

Jun Nakamura

Yoko Asakura

Megumi Shimaoka

Takayuki Asahara

Katsuya Seguro

Yoshiyuki Kumazawa

Yukiharu Uraoka

Yukiko Ono

Mikiko Yamaguchi

Yasuaki Ishikawa

Seizaburo Shiraga

Ichiro Yamashita

Bin Zheng

Noriko Murayama

Reiko Hirai

Yuri Miyata

Manami Oba

Kohichiro Sano

A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

Method for producing RNA

The present invention provides means useful for making devices, materials and the like that are excellent in photocatalytic activity, electric property or the like. Specifically, the present invention provides a fusion protein comprising a polypeptide portion capable of forming a multimer having an internal cavity, and a first peptide portion capable of binding to a first target substance and a second peptide portion capable of binding to a second target substance; a multimer of the fusion protein; a complex comprising the multimer of the fusion protein; and the like.

Fusion protein

A method for producing an L-amino acid is described, which is characterized by culturing abacterium capable of producing the L-amino acid in a culture medium to produce and accumulate the L-amino acid in the culture medium and collecting the L-amino acid from the culture medium.

Method for production of L-amino acid

The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Use of phosphoketolase for producing useful metabolites

A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase &#x3b1;-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.

Microorganism producing an amino acid of the L-glutamic acid family and a method for producing the amino acid

A method for production of L-lysine is provided which includes the steps of cultivating a methanol-utilizing bacterium in a culture medium to produce and accumulate L-lysine in the culture medium and collecting the L-lysine from the culture medium, wherein the methanol-utilizing bacterium contains DNA encoding dihydrodipicolinate synthetase which is desensitized to feedback inhibition by L-lysine and DNA encoding a LysE protein that can enhance the excretion of L-lysine out of the methanol-utilizing bacterium, and the bacterium is modified so as to increase the intracellular activities of diaminopimelic acid dehydrogenase, diaminopimelic acid decarboxylase, dihydrodipicolinic acid reductase and aspartate-semialdehyde dehydrogenase.

Method for production of L-lysine using methanol-utilizing bacterium

L-amino acids such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, and L-cysteine are produced by culturing in a medium a bacterium having an L-amino acid-producing ability and wherein the bacterium has been modified so that the phosphotransacetylase activity is enhanced.

Method for producing L-amino acid

A DNA encoding a variant of a protein having a loop region and six hydrophobic helixes which is involved in excretion of L-lysine to outside of a cell is described, wherein the DNA encodes a mutant protein which does not contain the loop region that is present in the wild-type protein. The mutant protein facilitates excretion of L-lysine, L-arginine, or both to the outside of the cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium. Specifically, lysE24 is introduced into a methanol assimilating bacterium such asbacteria which results in improved L-amino acid productivity, especially production of L-lysine and L-arginine.

Method for producing L-lysine or L-arginine by using methanol assimilating bacterium

A DNA encoding for a mutant of LysE protein, or a homologous protein thereof, of a coryneform bacterium, wherein the mutant, when introduced into a methanol-assimilating bacterium imparts resistance to L-lysine analogue. The DNA encoding for a mutant of LysE protein, or a homologous protein thereof, is introduced into a methanol-assimilating bacterium to improve L-lysine and L-arginine productivity of the methanol-assimilating bacterium.

Method for producing L-amino acid using methylotroph

A DNA encoding a variant of a protein, the protein having a loop region and six hydrophobic helices and involved in secretion of L-lysine to the outside of a cell, wherein the DNA encodes a variant of a protein not containing the loop region and facilitates secretion of L-lysine, L-arginine or both of these L-amino acids to the outside of a methanol-assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into abacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.

Method for producing L-lysine or L-arginine by using methanol-assimilating bacterium

L-Arginine is produced by culturing a microorganism which has L-arginine producing ability and has been modified so that expression of lysE gene should be enhanced, such a microorganism further modified so that an arginine repressor should not function normally, or such a microorganism further modified so that intracellular activity of an enzyme in L-arginine biosynthetic pathway should be enhanced in a medium to produce and accumulate L-arginine in the medium and collecting the L-arginine from the medium.

Method for producing L-arginine

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Kawasaki, Japan

Hisashi Yasueda