Mitsubishi Petrochemical Company Limited

Research Association for Utilization of Light Oil

Hisashi Yamagata

Hideaki Yukawa

Masato Terasawa

Terukazu Nara

Yasurou Kurusu

Youichi Niimura

Michio Kozaki

Makoto Goto

Yukie Satoo

Makiko Fukushima

Yasukazu Uchida

Miki Ikuta

Makiko Kukushima

Masato Terasausa

A process for producing an NADH oxidase which comprises culturing alkalophilic, facultative anaerobic NADH oxidase-producing bacteria having the optimum growth pH in an alkaline region in medium and collecting said NADH oxidase from the culture; and a plasmid used for the said process.

Process for producing NADH oxidase

A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.

Cultivation of transformed microorganisms

Microorganisms of the genus Pseudomonas containing aspartate beta-decarboxylase at a high level are produced by culturing said microorganism in a medium supplemented with a chelating agent. L-Alanine can be efficiently produced from L-aspartic acid said microorganisms or the treated product thereof.

Process for culturing microorganisms of the genus Pseudomonas and

An aqueous solution containing fumaric acid is reacted in the presence of an aerobically cultured product of a microorganism belonging to the Brevibacterium genus and having resistance to .alpha.-aminobutyric acid, whereby L-malic acid is produced.

Method of preparing L-malic acid

Growing community of inventors

Toride, Japan

Hisashi Yamagata