Fujifilm Corporation

Fuji Photo Film Company, Limited

Hisashi Ohtsuka

Yoji Okazaki

Takayuki Katoh

Hitoshi Shimizu

Toshihito Kimura

Nobuhiko Ogura

Shu Sato

Hiroaki Hyuga

Tatsuo Fujikura

Morihito Ikeda

Koji Kuruma

Masayuki Naya

Hiroaki Yamamoto

Hiromi Ishikawa

Naoki Murakami

Kiichi Kato

Shinji Mitsumoto

A magnetic binding substance, which is a first binding substance that specifically binds with a target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and surfaces modified with functional groups that exhibit polarity within a liquid sample, attached thereto, and a labeling binding substance, which is a second binding substance that specifically binds with the target substance having photoresponsive labels attached thereto, are mixed with the liquid sample such that binding reactions occur. A magnetic field is generated within a sample cell, to draw the magnetic binding substance to a local region. Excitation light is irradiated only onto a predetermined region including the local region while the magnetic binding substance is drawn to the local region, causing the photoresponsive labels present therein to generate optical signals. The optical signals are detected.

Detecting method and dielectric particles containing magnetic material employed in the detecting method

In a device for mass spectrometry, an analyte contained in a sample is desorbed from a surface of the device by irradiating the sample in contact with the surface with measurement light. The device includes a micro-structure having a plurality of metal bodies on a surface of a substrate, and the plurality of metal bodies have sizes that can excite localized plasmons by irradiation with the measurement light. Further, the device includes an initiator fixed at least to a part of a surface of the micro-structure.

Device for mass spectrometry, and mass spectrometry apparatus and method

A fluorescence detecting method utilizes surface plasmon enhancement. An electric field enhancing field is caused to be generated at a detecting portion that includes a metal film provided on a surface of a dielectric prism. Fluorescence emitted by fluorescent labels, which are attached to a detection target substance, due to the excitation effect of the electric field enhancing field is detected by a photodetector. During the detection, a plurality of fine metal particles are dispersed on the detecting portion.

Fluorescence detecting method

A sensor-chip includes a sensor-portion having at least a metal-layer deposited on a surface of a dielectric-plate. A fluorescent-label binding-substance in an amount corresponding to the amount of a detection-target-substance in a liquid-sample binds onto the sensor-portion. The amount of the detection-target-substance is detected based on the amount of light generated by excitation of a fluorescent-label in the fluorescent-label binding-substance. A fluorescent-substance the charge state of which changes in the liquid-sample according to the pH of the liquid-sample, and which contains a plurality of fluorescent-dye-molecules enclosed by a material that transmits fluorescence output from the fluorescent-dye-molecules, is used as the fluorescent-label. With the fluorescent-label binding-substance bound to the sensor-portion, the fluorescent-label is attracted to the sensor-portion by adjusting the pH of the sample-liquid to neutralize the charge state of the fluorescent-substance, thereby attracting the fluorescent-label onto the sensor-portion. In this state, the amount of the detection-target-substance is detected.

Detection method, detection apparatus, and sample cell and kit for detection

An immunochromatoassay method that allows high detection sensitivity measurement. The method including the steps of: permeating an analyte solution that includes a visibly labeled second binding substance that specifically binds to a detection target substance into a test area of a chromatography medium provided with a first binding substance that specifically binds to the detection target substance, simultaneously with or after the permeation of the analyte solution into the test area, permeating a visual recognition aid solution into the chromatograph medium, the solution having a refractive index whose refractive index difference &#x394;n from that of the chromatograph medium is &#x2212;0.1&#x2266;&#x394;n&#x2266;0.1, and visually observing the test area while the visual recognition aid solution is permeated in the test area.

Immunochromatoassay method and immunochromatoassay kit

Reference data are subtracted from corresponding actual measurement data, and unprocessed binding quantity data, each of which represents a quantity of binding of a ligand and each of analytes, are thereby acquired. Calculation is made to find a relationship between the reference data and the unprocessed binding quantity data acquired in cases where the binding does not occur. In accordance with the relationship, variation components, which are contained in the unprocessed binding quantity data, are calculated. The variation components are subtracted from the corresponding unprocessed binding quantity data, and processed binding quantity data are thereby acquired. The processed binding quantity data are utilized for extracting an analyte capable of binding with the ligand.

Screening method and apparatus

A fluorescence sensor is constituted by: a light source, for emitting excitation light of a predetermined wavelength; a dielectric block, formed of a material that transmits the excitation light; a metal film, formed on a surface of the dielectric block; a non flexible film of a hydrophobic material, formed on the metal film at a film thickness within a range of 10 to 100 nm; a sample holding portion, for holding a sample such that the sample contacts the non flexible film; an incident optical system, for causing the excitation light to enter the interface between the dielectric block and the metal film through the dielectric block such that conditions for total internal reflection are satisfied; and fluorescence detecting means, for detecting fluorescence emitted by a substance within the sample, which is excited by evanescent waves that leak from the interface when the excitation light enters the interface.

Surface plasmon enhanced fluorescence sensor and fluorescence detecting method

A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

Sensor unit and assay method of assay in utilizing attenuated total reflection

A fluorescence sensor comprises a sensor section for collecting a fluorescent substance, which acts to represent presence of a substance to be detected in a sample, and an exciting light source, which produces exciting light for exciting the fluorescent substance to produce fluorescence. Besides the exciting light source, at least one different non-exciting light source is located for irradiating different non-exciting light, which varies in wavelength from the exciting light and which is substantially free from capability of exciting the fluorescent substance, to the sensor section.

Fluorescence sensor

A surface plasmon resonance assay apparatus is loaded with a sensor unit. A sensing surface of a thin film detects reaction of a sample. A dielectric prism is overlaid with the thin film to constitute an interface. A reflection angle upon occurrence of attenuated total reflection of the illuminating light is changeable according to reaction of the sample on the sensing surface. Protecting panels are disposed to face outer surfaces of the prism, for covering and protecting at least partially the outer surfaces. A first window in one of the protecting panels is positioned on a path of the illuminating light traveling for incidence on the interface, for passing the illuminating light. A second window in one remaining protecting panel is positioned on a path of the illuminating light traveling upon reflection by the interface, for passing the illuminating light.

Sensor unit for assay in utilizing attenuated total reflection

A base plate for mass spectrometry analysis is disclosed, which is used in a method in which a substance immobilized on a surface of the base plate is desorbed from the surface by application of laser light thereto and the ion of the desorbed substance is captured for mass spectrometry analysis. The base plate includes, on at least a portion of the surface thereof, a roughened metal surface capable of exciting local plasmon when exposed to laser light. The roughened metal surface is formed, for example, by forming numerous micropores in a surface of an alumina layer and filling gold particles in the micropores. Each gold particle has a head portion having a size larger than a diameter of the micropore and projecting from the surface of the alumina layer. Use of this base plate allows use of lower-power laser light.

Base plate for use in mass spectrometry analysis, and method and apparatus for mass spectrometry analysis

A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio)chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

An object of the present invention is to provide a method for measuring the dissociation rate coefficient (Kd) by surface plasmon resonance analysis without measuring the theoretical maximum amount of binding (Rmax). The present invention provides a method for measuring the dissociation rate coefficient (Kd) of the reaction between an analyte molecule immobilized on a metal surface and a molecule that interacts with the analyte molecule by assaying changes in surface plasmon resonance signals using a surface plasmon resonance measurement device, wherein the signal and the slope of the dissociation curve of the surface plasmon resonance signal curves, or the signal ratio are used to calculate the dissociation rate coefficient (Kd).

Method for measuring dissociation rate coefficient by surface plasmon resonance analysis

A solid-state laser crystal constituting a laser-diode-excited solid-state laser apparatus or an optical fiber constituting a fiber laser apparatus or fiber laser amplifier is doped with one of Ho, Sm, Eu, Dy, Er, and Tbso that a laser beam is emitted from the solid-state laser crystal or the optical fiber, or incident light of the fiber laser amplifier is amplified, by one of the transitions fromStoI, fromStoI, fromGtoH, fromGtoH, fromFtoH, fromDtoF, fromFtoH, fromFtoH, fromStoI, fromHtoI, and fromDtoF. The above solid-state laser crystal or optical fiber is excited with a GaN-based compound laser diode.

[object Object]

An object of the present invention is to provide a method for measuring a rate coefficient by surface plasmon resonance analysis with excellent accuracy, reliability, and cost performance that can improve the assay accuracy and simplify the apparatus. The present invention is provides a method for determining the adsorption rate coefficient (Ka) and the dissociation rate coefficient (Kd) of the reaction between an analyte molecule immobilized on a metal surface and a molecule that interacts with the analyte molecule by measuring changes in surface plasmon resonance signals using a surface plasmon resonance measurement device and performing fitting to a signal curve obtained by such measurement, wherein fitting is performed to the signals from which data obtained at the time of liquid exchange and data obtained at a given time point thereafter have been removed, and that an algorithm is adopted wherein the fitted curve passes through a point representing a baseline value obtained at the time of liquid exchange or immediately therebefore.

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Kaisei-machi, Japan

Hisashi Ohtsuka