Sapporo Breweries Limited

Hisao Kuroda

Naohiko Hirota

Kazutoshi Ito

Kazuyoshi Takeda

Takafumi Kaneko

Hirotaka Kaneda

Kiyoshi Takoi

Makoto Kihara

A barley plant including a mutant LOX-1 protein and that is deficient in lipoxygenase activity.

Barley lipoxygenase 1 gene, selection method for barley, materials for malt alcoholic beverages and method for production of malt alcoholic beverages

A selection method for barley lipoxygenase-1 deficient barley, comprising a step of distinguishing the barley lipoxygenase-1 deficient barley by whether or not the guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene is mutated to a different base; and a method for production of malt alcoholic beverages using a material for malt alcoholic beverages derived from barley obtained by the selection method.

Barley Lipoxygenase 1 Gene, method of selecting barley variety, material of malt alcoholic drinks and process for producing malt alcoholic drink

The present invention provides new gene expression regulatory DNA. This gene expression regulatory DNA comprises a promotor region derived from the barley D-hordein gene enabling the expression of a desired gene, and a regulatory region for regulating the expression of said gene based on said promoter region. This regulatory region consists at least of an activating region to activate the expression of said gene and a suppressing region to suppress the same. Use of this gene expression regulatory DNA enables controlling as desired the expression of gene within a plant cell such as barley.

Gene expression regulatory DNA, expression cassette, expression vector

cDNA derived from the ARF gene that is actively expressed in barley seeds, roots and callus, but is highly suppressed or almost not expressed at all in leaves, was cloned. Next, the upstream region of the ARF gene was cloned, its promoter activity was verified, and a gene expression vector using the expression region of this gene was produced.

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Yaizu, Japan

Hisao Kuroda