Ajinomoto Co., Ltd.

Hisao Fukuda

Yoshiyuki Takahara

Takami Maekawa

Akira Mitsui

Shigeru Yamanaka

Masayo Date

Toshio Kato

Tomohisa Okutsu

Naoko Tsuyoshi

Kaoru Kubota

Fumihiko Yokoya

An isolated polypeptide comprises the amino acid sequence of SEQ ID NO: 11, wherein the expression of said polypeptide is significant lower specifically in human livers with hepatitis compared with that in human health livers. The detection of said polypeptide expression in human liver can be used as a diagnosis for human hepatitis.

Polypeptides, use thereof and process for producing the same

In gene expression frequency analysis, a vector primer to which each cDNA is ligated is formed by synthesizing the cDNA from each mRNA using by a vector primer having a poly(T) sequence; each cDNA sequence is converted to a tag on the vector primer; a concatemer is formed by ligating the obtained tags via a sequence that enables recognition of ends of the tags; and the nucleotide sequence of the concatemer is analyzed.

Method for analyzing gene expression frequency

The present invention provides a method for producing a condiment with decreased animal smell and which has increased beefwise taste and greater body flavor and complex flavor. The condiment is produced by blending therein denatured meat and/or the extract thereof, wherein the denatured meat is obtained by salting livestock meat, washing and then allowing microorganisms to ferment and ripen the meat.

Process for producing a condiment

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Kawasaki, Japan

Hisao Fukuda