Nippon Kayaku Kabushiki Kaisha

Hisako Takagi

Takao Yokoyama

Reiko Machida

Yoshihiko Umegae

Yayoi Irie

Toshio Tanabe

Kan Saiga

Kyuichi Nemoto

Isamu Koyama

Takako Mae

Tadaki Yasumura

Atsuko Isoda

A biosensor for electrochemically measuring a sample may include a first member made of an insulating material, an electrode system including a working electrode and a counter electrode formed on the first member, a second member fixed over the first member, a sample flow channel provided between the first member and the second member, a hydrophilic section provided on at least a part of the internal surface of the sample flow channel and extending from a first end near the electrode system to a second end on the opposite side, and a flow stop area provided on a section adjacent to the second end on the first member or on the internal surface of the sample flow channel.

Biosensor

The biosensor has an electrode system for electrochemically measuring 1,5-anhydroglucitol (1,5-AG) and a reagent layer formed on said electrode system. The reagent layer contains an enzyme for measuring 1,5-anhydroglucitol, phenothiazine compounds, a stabilizer selected from a group of compounds comprising metal salts, organic acids, and amino acids, and an acidic polymer compound as an optional ingredient. The biosensor has excellent storage stability and can electrochemically measure 1,5-anhydroglucitol unaffected by the hematocrit contained in a whole blood sample.

Biosensor for electrochemical measurement of 1,5-anhydroglucitol, and measuring method and measuring kit using the same

By a method for measuring 1,5-anhydroglucitol, comprising the steps of: eliminating or converting glucose interfering with the measurement of 1,5-anhydroglucitol and/or a derivative thereof beforehand; and measuring 1,5-anhydroglucitol performed thereafter, wherein such glucose and/or a derivative thereof are/is eliminated or converted in whole blood as such without performing blood cell separation, an enzyme for measuring 1,5-anhydroglucitol is allowed to act on without performing blood cell separation, and 1,5-anhydroglucitol is electrochemically measured, it becomes possible to measure 1,5-anhydroglucitol using a small amount of whole blood without resort to a centrifuge or the like. Accordingly, this measurement method can be applied to rapid measurement of 1,5-anhydroglucitol at bedside or in a medical examination room or to home self-measurement thereof by a patient.

Method for electrochemically measuring 1,5-anhydroglucitol in whole blood

A method for detecting the onset of chronic rejection at an early stage after organ transplantation, characterized by assaying nitrogen trioxide (NO,), matrix metalloproteinase (MMP)-2 or the precursor thereof in body fluids drawn out of body.

Method for examining chronic rejection reactions following organ transplantation and method for determining urine components

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Takasaki, Japan

Hisako Takagi