Kyowa Medex Co., Ltd.

Kumamoto Health Science University

Hiroyuki Sugiuchi

Kaneto Uekama

Tetsumi Irie

Eiko Shutoh

Kazuhito Miyauchi

Susumu Ohsawa

Akira Miike

Toshio Tatano

Norihiko Kayahara

Yuki Katayama

Kazumi Matsushima

The present invention provides a method for simply and precisely measuring cholesterol in an HDL subfraction contained in a sample. This is a method for measuring cholesterol in HDL3 contained in a sample, which comprises reacting a sample with (1) a combination of a cholesterol ester hydrolase and a cholesterol oxidase or (2) a combination of a cholesterol ester hydrolase, an oxidized coenzyme and a cholesterol dehydrogenase in an aqueous medium containing: (a) a divalent metal salt; (b) an alkali metal salt selected from the group consisting of a sulfate, a nitrate, a carbonate, an acetate and a halide; and (c) dextran sulfate or a salt thereof, and measuring a substance formed or consumed in the reaction without separating and removing lipoproteins other than HDL3.

Method for measuring cholesterol in HDL subfraction, and reagents and kit therefor

The present invention provides a method for the quantitative determination of cholesterol in low density lipoproteins and a reagent kit for use therein. The present invention also provides a method for continuous fractional determination of cholesterol in high density lipoproteins and cholesterol in low density lipoproteins and a reagent kit for use therein, as well as a method for continuous fractional determination of cholesterol in high density lipoproteins and total cholesterol and a reagent kit for use therein.

Methods for fractional quatification of cholesterol in lipoproteins and quantification reagents

Method for determination of cholesterol in low-density lipoprotein or

Provided is a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises reacting an HDL-containing sample with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase in the presence of a reagent for aggregating lipoproteins- except HDL, and determining the amount of hydrogen peroxide or reductive co-enzyme formed therein.

Method of determining the amount of cholesterol in a high-density

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Kumamoto, Japan

Hiroyuki Sugiuchi