Japan Science and Technology Agency

The University of Tokyo

Sysmex Corporation

Osaka University

Fukoku Co., Ltd.

Hiroyuki Noji

Hiroaki Suzuki

Ryota Iino

Kazuhito Tabata

Suguru Araki

Rikiya Watanabe

Yoshihiro Minagawa

Lisa Yamauchi

Yasuteru Urano

Hiroaki Suga

Shoji Takeuchi

Mako Kamiya

Jun Ohta

Kenji Akama

Takashi Tokuda

Kiyotaka Sasagawa

Toshihiko Noda

Daishi Fujita

Takao Ono

Soo Hyeon Kim

Yoshimi Matsumoto

Kohei Hayama

Shouichi Sakakihara

Kunihiko Nishino

Naoki Soga

Akihito Yamaguchi

As a technique for detecting an influenza virus with an improved accuracy, there is provided a method for detecting an influenza virus in a biological sample by using a first probe, which is decomposed by an influenza virus-derived neuraminidase and a bacterium-derived neuraminidase to generate an optically detectable signal, and a second probe, which is decomposed by the bacterium-derived neuraminidase to generate an optically detectable signal and not decomposed by the influenza virus-derived neuraminidase.

Method and kit for detecting influenza virus, and method for diagnosing influenza virus infection

There is provided a method of detecting a microscopic body stored in a plurality of receptacles formed separately from each other. The method, which is provided as a technique for enclosing a to-be-detected substance such as nucleic acid, protein, virus, and cell by means of a simple operation in droplets of an extremely small volume and enabling highly sensitive detection, includes the steps of (1) introducing a solvent into a space between a lower layer part in which the receptacles are formed and an upper layer part facing a surface of the lower layer part in which surface the receptacles are formed, wherein the solvent contains the microscopic body; (2) introducing gas into the space to form a droplet of the solvent in the receptacles, wherein the droplet contains the microscopic body; and (3) detecting the microscopic body present in the droplet optically, electrically, and/or magnetically.

Microscopic body detection method and microscopic body detection device

Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).

Method for detecting enzymatic reaction product

Disclosed is an analyte detection method, including detecting an analyte in such a state that a complex composed of the analyte in a sample of interest, a particle capable of bonding to the analyte and a trapping substance capable of bonding to the analyte is formed on a substrate, wherein the analyte is detected on the basis of an index associated with a behavior of the particle.

Analyte detection method

Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.

Microscopic body enclosing method, microscopic body detection method, and microscopic body detection device

Provided is a method for detecting pathogenic microorganisms in a biological sample, which is a technique that can be used to perform high-sensitivity detection of pathogenic microorganisms, such as influenza virus, etc.

Method and kit for detecting pathogenic microorganism

A high-density micro-chamber array has a translucent flat substrate, a hydrophobic layer in which a plurality of micro-chambers are provided, and a lipid bilayer membrane formed in each of the openings of the micro-chambers, wherein an electrode is provided in each of the micro-chambers, and when the side of the substrate on which the hydrophobic layer is provided is directed upward, the micro-chamber array is configured such that with at least one of the following A) and B) being met, light entering the substrate from below is transmitted through the substrate and penetrates into the micro-chambers' interiors, and light entering the substrate from the micro-chambers' interiors is transmitted through the substrate and escapes toward below the substrate. A) The electrode is provided on an inner side surface of each of the micro-chambers. B) The electrode is transparent and provided on a bottom surface of each of the micro-chambers.

High-density micro-chamber array and measurement method using same

This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent () containing beads (),(') into a space () between (a) a lower layer section () including a plurality of receptacles () each of which is capable of storing only one of the beads (),(&#x2032;) and which are separated from each other by a side wall () having a hydrophobic upper surface and (b) an upper layer section () facing a surface of the lower layer section () on which surface the plurality of receptacles () are provided; and (ii) a step of introducing a hydrophobic solvent () into the space (), the step (ii) being carried out after the step (i).

Method for detecting target molecule

A material film is formed as a thin film having a thickness of 1 &#x3bc;m on a surface of a glass substrate. A plurality of micro-chambers having a diameter of 5 &#x3bc;m are formed in the material film to be arrayed at a high density. The respective chambers filled with an aqueous test solution have openings that are liquid-sealed by a lipid bilayer membrane to provide a high-density micro-chamber array. Significant downsizing of the micro-chambers enhances a change in concentration by a reaction of one biomolecule in the chamber and thereby increases the detection sensitivity. In the configuration that a large number of micro-chambers are formed at a high density, even in the case of an extremely slow reaction of the biomolecule, the reaction proceeds in any of the chambers. This configuration accordingly enables the reaction of the biomolecule to be detected with high sensitivity.

High-density microchamber array and manufacturing method thereof

As a technique for efficiently sealing many substances, such as beads, nucleic acid, protein, virus, cells, and lipid membrane complex, into an array, the present invention provides a method for sealing a substance, including: (i) a step of introducing a first solvent containing a substance on a substrate on which a plurality of receptacles capable of storing the substance are formed separated from each other by a side wall; and (ii) a step of introducing a second solvent having a greater specific gravity than that of the first solvent onto the first solvent, the step (ii) being carried out after the step (i).

Substance sealing method and target molecule detecting method

This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent () containing beads (),() into a space () between (a) a lower layer section () including a plurality of receptacles () each of which is capable of storing only one of the beads (),() and which are separated from each other by a side wall () having a hydrophobic upper surface and (b) an upper layer section () facing a surface of the lower layer section () on which surface the plurality of receptacles () are provided; and (ii) a step of introducing a hydrophobic solvent () into the space (), the step (ii) being carried out after the step (i).

Kit for sealing beads and detecting target molecule

A technique for efficiently sealing many substances, such as beads, nucleic acid, protein, virus, cells, and lipid membrane complex, into an array is provided. The present invention provides a method for sealing a substance, including: (i) a step of introducing a first solvent containing a substance on a substrate on which a plurality of receptacles capable of storing the substance are formed separated from each other by a side wall; and (ii) a step of introducing a second solvent having a greater specific gravity than that of the first solvent onto the first solvent, the step (ii) being carried out after the step (i).

Method for sealing substances, method for detecting target molecule, array, kit, and target molecule detection device

There are provided a detector and a detection method capable of detecting a biological sample with high sensitivity. A detector is provided with a microchamber array including a plurality of storage sections to be filled with a hydrophilic solvent containing a biological sample; and an image sensor in which picture elements are disposed corresponding to the storage sections, wherein the microchamber array includes a flow channel in communication with openings of the storage sections; a hydrophobic solvent supply unit arranged in continuity with the flow channel; and a through-hole which allows the hydrophilic solvent to enter and exit the flow channel, and the hydrophobic solvent supply unit flows a hydrophobic solvent into the flow channel by an externally-applied force.

Detector and detection method

The present disclosure describes a method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug. The inspection method of the present disclosure is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having flow channels, including: incubating a mixture of the antimicrobial drug and a suspension to be inspected in the flow channels of the micro-device; and detecting bacteria or fungi derived from the suspension to be inspected in an observation area of the flow channels of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the suspension to be inspected in the observation area by a microscope or the like.

Growing community of inventors

Tokyo, Japan

Hiroyuki Noji