Kaneka Corporation

Hiroyuki Kanamaru

Makoto Ueda

Yoshihiko Yasohara

Hirokazu Nanba

Ryuuji Miki

Shigeru Kawano

Masutoshi Nojiri

Osamu Odawara

Akiko Nishi

Teruaki Takesue

Hironori Nanba

Takeyuki Tsuchidate

The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32&#xb0; C. or higher abacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing thebacterium at a temperature lower than 32&#xb0; C. after the high-temperature culture step.

Method for producing recombinant proteins using recombinant brevibacillus

An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.

Vector, a transformant and a method to produce a polypeptide having activity to selectively hydrolyze a (R)-tropic acid amide in a racemic mixture

The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.

Method for production of L-amino acid

The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.

Amino acid dehydrogenase, and process for producing L-amino acid, 2-oxo acid or D-amino acid

The present invention relates to novel D-amino acid oxidase isolated and purified from, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.

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Takasago, Japan

Hiroyuki Kanamaru