Takara Shuzo Co., Inc.

Takara Bio Inc.

Hiroyuki Izu

Ikunoshin Kato

Makoto Ito

Mutsumi Sano

Toyohisa Kurita

Nozomu Okino

Noriyuki Sueyoshi

Katsuhiro Kita

Masanori Fujita

Yoshiya Izumi

Susumu Mitsutake

Yoko Kurome

Masanori Mitta

A process for producing lysosphingolipids by using an enzyme capable of specifically hydrolyzing the acid amide bond between a sphingoid and a fatty acid in a sphingolipid, characterized in that the enzyme reaction is carried out in a two-phase system liquid reaction mixture containing at least an organic solvent forming an aqueous phase and a separation phase in the presence of, if desired, a surfactant.

Process for producing lysosphingolipids

The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

Sphingolipid ceramide N-deacylase, methods for producing sphingolipids and sphingolipid derivatives, and sphingolipid ceramide N-deacylase gene

Sphingolipid ceramide N-deacylase, methods for producing sphingolipids and sphingolipid derivatives, and spingolipid ceramide N-deacylase gene

An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activator activity or functionally equivalent variants thereof; and a method for producing a polypeptide possessing endoglycoceramidase activator activity or functionally equivalent variants thereof by gene recombinant technology.

Gene encoding endoglycoceramidase activator

An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof, and a method for producing a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof by gene recombinant technology.

Gene encoding endoglycoceramidase

An almond N-glycosidase gene and genes associated therewith are described. Vectors integrated such genes therein, recombinant microorganisms transformed with said vectors, and a process for preparing the almond N-glycosidase using said recombinant microorganisms are also described.

Almond N-glycosidase gene

Growing community of inventors

Shiga, Japan

Hiroyuki Izu