Hiroshima University

Hiroshima Industrial Promotion Organization

Hiroyuki Horiuchi

Haruo Matsuda

Shuichi Furusawa

Yusuke Yamashita

Mikiharu Nakano

Masaki Nishimoto

A chicken embryonic stem cell is established, which stably has pluripotency and an ability of being differentiated into a germ cell. For evaluating on whether or not the chicken embryonic stem cell can be applied to genetic modification technique, detection is made on a protein which serves as an indicator of the ability of being differentiated into a germ cell. This provides (i) a chicken embryonic stem cell applicable to genetic modification technique and (ii) a method for evaluation of the chicken embryonic stem cell.

Method of determining chicken embryonic stem cells

The present invention is to provide a chicken LIF gene. Based on this genetic information, LIF protein derived from the chicken can be stably supplied and it solves the problems of the creation of transgenic chickens in the past. In addition, the present invention provides not only transgenic chickens for testing purposes but also supplies the first practical transgenic stock animals. The present invention relates to leukemia inhibitory factor (LIF) shown in sequence No. 2, and the gene that encodes thereof shown in sequence No. 1, and a manufacturing method of chicken LIF. In addition, the present invention pertains to a differential preventer of the chicken differentiable cell, and a method of chicken differential prevention and a culturing method for the chicken differentiable cell using thereof, and a medium comprising thereof.

Medium comprising chicken leukemia inhibitory factor (LIF)

Method of preventing differentiation of a chicken differentiable cell using chicken leukemia inhibitory factor (LIF)

Oligonucleotides of a chicken leukemia inhibitory factor (LIF) gene

Nucleic acid encoding chicken leukemia inhibitory factor (LIF)

Chicken leukemia inhibitory factor (LIF)

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Hiroshima, Japan

Hiroyuki Horiuchi