Toyota Jidosha Kabushiki Kaisha

Hiroyuki Enoki

Satoru Nishimura

Takeo Sakaigaichi

Tatsuro Kimura

Shoko Ishikawa

Taiichiro Hattori

Takayoshi Terauchi

Aya Murakami

Yoshifumi Terajima

Masayoshi Muramatsu

Susumu Yui

Masanori Honjo

Hiroaki Koishihara

Yoshie Takeuchi

Shoko Tsuzuki

Minoru Inamori

Masaaki Mori

Takehiko Shimada

Minoru Tanaka

Yuji Noguchi

Naoko Tsurumaru

Momoe Suitou

Makoto Umeda

Taichiro Hattori

Momoe Suito

Katsuki Adachi

Yusuke Tarumoto

Tsukasa Nunome

Yusuke Ueta

Michiko Hayano

When preparing a DNA library via a nucleic acid amplification reaction using a random primer in a convenient and highly reproducible manner, amplification of DNA fragments derived from the chloroplast genome is reduced to a significant extent. A random primer comprises oligonucleotides selected from oligonucleotides group represented by TAAGAGACAGNN excluding those in which 2 bases at the 3' terminus are TG and oligonucleotides group represented by TAAGAGACAGNNN excluding those in which 3 bases at the 3′ terminus are TGC.

Set of random primers and method for preparing DNA library using the same

This invention is intended to evaluate smut resistance with higher accuracy using a marker associated with sugarcane smut resistance, which consists of a continuous nucleic acid region existing in a region between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 6, a region between the nucleotide sequence as shown in SEQ ID NO: 135 and the nucleotide sequence as shown in SEQ ID NO: 143, or a region between the nucleotide sequence as shown in SEQ ID NO: 144 or 145 and the nucleotide sequence as shown in SEQ ID NO: 151 of a sugarcane chromosome.

Marker associated with smut resistance in plant belonging to genusand use thereof

This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5'-CAAGCAGAAGACGGCATACGAGAT-N-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3&#x2032;, wherein Nindicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Primer for next generation sequencer and a method for producing the same, a DNA library obtained through the use of a primer for next generation sequencer and a method for producing the same, and a DNA analyzing method using a DNA library

This invention intends to develop many DNA markers for a plant of the genusand detect powdery mildew resistance with high precision by using the many DNA markers. The marker associated with powdery mildew resistance in a plant of the genuscomprises a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 19 in the chromosome of the plant of the genus

Marker associated with powdery mildew resistance in plant of genusand use thereof

This invention is intended to develop many DNA markers in plants of the genusand to evaluate anthracnose resistance with high accuracy with the use of such many DNA markers. The markers associated with anthracnose resistance in plants of the genuseach comprise a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 10 in the chromosome of a plant of the genus

Marker associated with anthracnose resistance in plant of the genusand use thereof

This invention intends to develop many DNA markers for a plant of the genusand identify everbearing lines with high precision by using the many DNA markers. The marker associated with everbearing properties in a plant of the genuscomprises a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 5 in the chromosome of the plant of the genus

Marker associated with everbearing properties in plant of genusand use thereof

The invention provides a marker related to quantitative traits of a Gramineae plant, and in particular, stalk length. Such Gramineae stalk-length-related marker comprises a continuous nucleic acid region selected from a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 2 of a chromosome of the Gramineae plant.

Stalk-length-related marker derived from genome of wild-type sugarcane and use thereof

The present invention relates to a marker associated with resistance to smut which is a quantitative trait of sugarcane. Specifically, a marker associated with resistance to sugarcane smut, which consists of a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 14 or a different similar region, is provided.

Marker associated with resistance to smut in plant belonging to genus, and use thereof

Provided is a probe to be used in a DNA microarray having an excellent detection rate of a polymorphism such as SNP contained in genomic DNA. A method for designing a probe according to the invention includes the steps of: specifying one or more regions covering at least a part of fragments flanked by restriction enzyme recognition sites recognized by a restriction enzyme, contained in genomic DNA derived from an organism to be tested; and designing a probe for the specified one or more regions for detecting the fragment in the organism to be tested.

Method for designing probe in DNA microarray, and DNA microarray provided with probe designed thereby

[object Object]

According to the present invention, a sugarcane-stalk-sugar-content-related marker linked to a sugarcane quantitative trait is provided. Such marker is a sugarcane-stalk-sugar-content-related marker, which comprises a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 8 or a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 9 and the nucleotide sequence shown in SEQ ID NO: 16.

Sugarcane-stalk-sugar-content-related marker and the use thereof

It is an object of the present invention to provide a method for identifying the variety/line of a plant of the genuswith the use of novel DNA markers that allow high-precision identification of a wide range of varieties/lines of plants of the genus. A method for identifying the variety/line of a plant of the genus, comprising using a simple sequence repeat polymorphism in at least one DNA sequence selected from SEQ ID NOS: 1 to 12 is provided.

Growing community of inventors

Okazaki, Japan

Hiroyuki Enoki