Meiji Seika Pharma Co., Ltd.

Mitsui Chemicals Crop & Life Solutions, Inc.

Hiroyuki Anzai

Kentaro Yamamoto

Masaaki Mitomi

Kazuhiko Oyama

Mariko Tsuchida

Naomi Sumida

Kimihiko Goto

Koichiro Murashima

Sato Aihara

Kouji Yanai

An isolated novel polynucleotide comprising a nucleotide sequence encoding at least one polypeptide involved in biosynthesis of pyripyropene A, a recombinant vector comprising the polynucleotide and a transformant comprising the polynucleotide are disclosed. By the present invention, a pyripyropene A biosynthetic gene useful for production of a novel pyripyropene analog, improvement of productivity of a pyripyropene A-producing bacterium, production of an insecticidal agent for microorganisms, creation of a plant resistant to insect pests or the like are provided.

Pyripyropene a biosynthetic gene

There is provided a method for culturing a microorganism in which a particular polynucleotide or a recombinant vector comprising it/them is introduced with an intermediate compound necessary for biosynthesis of pyripyropene A. The method of the present invention allows for the production of pyripyropene.

Method for producing pyripyropene

There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.

Nucleic acid construct comprising pyripyropene biosynthetic gene cluster and marker gene

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Ibaraki-ken, Japan

Hiroyuki Anzai