Nippon Paper Industries Co., Ltd.

Sanwa Kagaku Kenkyusho Co., Ltd.

Hiroyasu Ebinuma

Koichi Sugita

Etsuko Matsunaga

Takahito Jomori

Yuji Hayashi

Akira Tashita

Saori Kasahara

Fumio Takaiwa

Takehide Kasahara

Akiyoshi Kawaoka

Yukari Kobara

Hiroshi Yasuda

Mikiko Yamakado

Mika Kaneda

Mikiko Uesugi

Humio Takaiwa

Ayako Todate

The present invention is regarding plants and plant storage organs thereof in which GLP-1 derivatives are accumulated, and methods of producing them. The transgenic plants and plant storage organs thereof accumulate tandem repeated GLP-1 derivatives cleavable with intestinal digestive enzyme to monomeric molecules and are produced by methods comprising: integrating into vectors linked DNAs which comprise tandem repeated DNAs encoding the GLP-1 derivative with trypsin resistance in which the amino acid in the 26th position is Gln, the amino acid in the 34th position is Asn or Asp, and C-terminal consists of Arg or Lys to produce monomeric molecules; introducing the vectors into plant cells; and redifferentiating the obtained transformants. The edible transgenic plants and plant storage organs are useful for treating diabetes and can be ingested by diabetic patients.

Plant and plant storage organ having GLP-1 derivative accumulated therein and method of producing the same

The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.

GLP-1 derivative

A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme which synthesizes auxin from an auxin precursor; (B) culturing the plant cell into which the genes are introduced by the vector, in the presence of an auxin precursor and/or an analogue thereof to thereby prepare a redifferentiated tissue, and detecting and selecting the redifferentiated tissues; and (C) culturing the redifferentiated tissue selected in (B) to redifferentiate a plant individual, and a vector for gene introduction into a plant, which comprises: a desired gene, and a selectable marker gene comprising an indoleacetamide hydrolase, iaaH, gene and an isopentenyl transferase, ipt, gene and being free of an tryptophan monooxygenase, iaaM, gene.

Method for efficiently producing transgenic plant using auxin precursor

A vector for introducing a gene into a plant, wich comprises a desired gene, and a plant hormone signal transduction gene as a selectable marker gene.

Vector for introducing a gene into a plant using a selectable marker

The present invention relates to a vector for introducing a desired gene into a plant, wherein a selectable marker gene introduced into a plant cell along with a desired gene is optionally removable from the DNA such as chromosome or the like where it exists and functions, then disappeared the function thereof after its expression, and the expression of the selectable marker gene and the disappearance of the function thereof are detectable by morphological change of the tissue derived from the plant cell into which the selectable marker gene is introduced. Also, the present invention constitutes a vector using a morphological abnormality induction gene as a selectable marker gene, while putting a removable DNA element under control of an inducible promoter, wherein the morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein a desired gene is positioned such that it does not behave integrally with the removable DNA element.

Vector for gene transfer into plant allowing optional deletion of marker gene

The present invention relates to an isolated and purified DNA having a nucleotide sequence which comprises SEQ ID NO:1, and encodes a transcription factor controlling a phenylpropanoid biosynthesis pathway; a recombinant vector comprising the DNA; the recombinant vector, further comprising a promoter to which the DNA is operably fused; the recombinant vector, wherein the DNA is operably fused to the promoter in the sense or antisense direction; a plant cell into which the DNA has been introduced; a plant regenerated from the plant cell; and an isolated and purified DNA which encodes a protein having the amino acid sequence of SEQ ID NO:2.

DNA encoding a transcription factor controlling phenylpropanoid biosynthesis pathway

A method for introducing a gene into a plant, which comprises introducing a gene into a plant cell using a vector containing an adventitious shoot redifferentiation gene as a selectable marker gene under the control of a light-inducible promoter, and a vector for introducing a gene into a plant, which comprises a desired gene, an adventitious shoot redifferentiation gene as a selectable marker gene under the control of a light-inducible promoter, and a removable DNA element, wherein the selectable marker gene is positioned such that it behaves integrally with the removable DNA element, and wherein the desired gene is positioned such that it does not behave integrally with the removable DNA element.

Method for introducing a gene into a plant using an adventitious bud redifferentiation gene under the control of a light-inducible promoter as a selectable marker gene, and vector for introducing a gene into a plant using the same

A vector for introducing a desired gene into a planet, which comprises the desired gene and at least one morphological abnormally induction (MAI) gene as a marker gene, or which comprises the desired gene, at least one MAI gene and a removable element. A method for producing a transgenic plant free from the influence of a marker gene. A method for multitudinously introducing desired genes into one plant.

Vector for introducing a gene into a plant, and methods for producing

A process for producing a plant is described, which comprises the step of culturing a tissue of a plant belonging to the genus Populus, the section Leuce, thereby regenerating the plant via an adventitious bud, wherein the adventitious bud is differentiated from the cultured tissue using an adventitious bud differentiation medium having nitrogen source concentrations of from 1 to 15 mM as ammonia-nitrogen and from 15 to 50 mM as nitrate-nitrogen.

Growing community of inventors

Tokyo, Japan

Hiroyasu Ebinuma