Sysmex Corporation

Other

Kawasaki Jukogyo Kabushiki Kaisha

Nagoya University

Maruyama, Atsushi

Hiroya Kirimura

Nobuyasu Hori

Shigeki Iwanaga

Seigo Suzuki

Masayoshi Seike

Yukio Iwasaki

Junichi Matsuoka

Yukihiko Kitano

Satoshi Ouchi

Kenji Akama

Eiji Mitsui

Ayato Tagawa

Yukiharu Uraoka

Yuichi Yasuda

Hiroyuki Asanuma

Yuzuru Takamura

Miyuki Chikae

Zheng Bin

A dispensing robot of an assay robot system includes a first contact surface oriented in a downward direction or an obliquely downward direction in a vertical direction and dispenses when the dispensing robot operates a pipette that allows a tip to be attached to a lower end of the pipette and the attached tip to be detached by pressing a tip ejector, the dispensing robot comprising: a robot arm having a hand to hold a pipette holding piece attached thereto; and a controller controls the robot arm such that the robot arm holds the pipette with the tip attached thereto by causing the hand to hold the pipette holding piece and that the robot arm is elevated and the tip ejector is pressed by bringing an upper end portion of the tip ejector of the held pipette into contact with the first contact surface from the downward direction.

Dispensing robot, method of controlling dispensing robot, and dispensing method

Disclosed is a method for detecting a test substance, including a first contact step of bringing the test substance, a capturing bodythat has binding properties to the test substance and is fixed to a substrate, and a capturing bodythat has binding properties to the test substance and contains a label into contact with each other in a liquid to arrange at least a part of the capturing bodyon the substrate; after the first contact step, a first dissociation step of dissociating a part of substances containing the capturing bodyfrom the substrate and releasing it into the liquid; after the first dissociation step, a second contact step of bringing the test substance, the capturing bodythat has binding properties to the test substance and is fixed to a substrate, and the capturing bodythat has binding properties to the test substance and contains a label into contact with each other in the liquid to arrange at least a part of the capturing bodyon the substrate; after the second contact step, a second dissociation step of dissociating a part of substances containing the capturing bodyfrom the substrate and releasing it into the liquid; and a detection step of comparing a label-derived signal arrangement pattern on the substrate after dissociation in the first dissociation step with a label-derived signal arrangement pattern on the substrate after dissociation in the second dissociation step and detecting signals at substantially the same position as a signal indicating the test substance.

Method for detecting test substance

Disclosed is a method of detecting a test substance contained in a sample using a working electrode and a counter electrode. The method comprises (A) forming a complex on the working electrode, the complex comprising the test substance and a metal particle; (B) washing the working electrode; (C) adding a measurement solution to the working electrode and measuring a current, voltage or electric charge from the metal on the working electrode in the measurement solution by electrochemical measurement; and (D) detecting the test substance in the sample based on a measurement result obtained in the step (C). In the method, the step (B) comprises washing the working electrode using a measurement solution having the same composition as the composition of the measurement solution used in the step (C). In the method, pH of the measurement solution is set, based on zeta potentials of the metal particle and the working electrode, to pH in which no electrostatic interaction occurs between the metal particle and the working electrode or pH in which an electrostatic repulsive force occurs between the metal particle and the working electrode.

Method of detecting test substance

The present invention relates to a method for amplifying a nucleic acid. More specifically, the present invention relates to a method for amplifying a nucleic acid in a light irradiation dependent manner under a substantially isothermal condition using a photo-responsive nucleic acid.

Method for amplifying nucleic acid

Disclosed is an analyte detection method for detecting an analyte contained in a biological sample, constituted by irradiating a light of a first peak wavelength on a complex containing an analyte and fluorescent substance which gives off light of a second peak wavelength of 450 nm or higher but not exceeding 900 nm when irradiated with light of the first wavelength of 190 nm or higher but not exceeding 350 nm, detecting the light of the second peak wavelength given off by the fluorescent substance when irradiated by light of the first peak wavelength, by a photodetector, wherein the photodetector has a quantum efficiency in the second peak wavelength that is two or more times the quantum efficiency in the first peak wavelength.

Analyte detection method, fluorescence detection method, and fluorescence detection apparatus using same

In order to provide a method of electrochemically detecting a target substance, a method of electrochemically detecting an analyte, and a detection set which have a theoretical advantage in the measurement sensitivity obtained by a conventional electrochemical detection method using a working electrode with a trapping substance immobilized, can reuse the working electrode, and can detect an analyte regardless of the size thereof, there is provided a method including: attracting the target substance containing a labeling substance in a liquid sample to a working electrode in which a trapping substance for trapping the target substance containing a labeling substance is not present; and electrochemically detecting the target substance containing a labeling substance.

Method for electrochemically detecting target substance, method for electrochemically detecting analyte, and detection set

The present invention provides a method for electrochemically detecting a target substance and a method for electrochemically detecting an analyte using a probe holding substrate with a probe for trapping a target substance or an analyte held on the substrate body as well as a test chip and a detection set using the above detection methods.

Method of electrochemically detecting target substance, method of electrochemically detecting analyte, test chip, and detection set

A detection device for detecting a test substance which is capable of detecting a test substance and a sample substance with high sensitivity, an electrode substrate, a working electrode, an inspection tip, a method of detecting a test substance, and a method of detecting a sample substance are provided in which a reflective part (reflective layer) is disposed on the working electrode so as to reflect excitation light emitted from a light source and passing through the working electrode toward the working electrode.

Detection device for detecting a test substance

A sample substance S is captured by a capture substancewhich is immobilized on a working electrode bodyto provide a method of electrochemically detecting a sample substance capable of detecting the sample substance with high sensitivity. A complex which includes, on a soluble carrier, a sample substance S and a labeled binderthat has a modified labeled substancecontaining a labeled substancebound to modified labeled substancecontaining a labeled substanceand a binderthat binds to the sample substance S, formed on the working electrode. The soluble carrieris dissolved, and the modified labeled substanceis attracted to the working electrode

Growing community of inventors

Kobe, Japan

Hiroya Kirimura