Shinwa Chemical Industries Ltd.

Eisai Company, Limited

Hiroya Fujima

Hiroo Wada

Jun Haginaka

Toshinobu Miwa

An optical isomer separating agent comprises a stationary phase in which the molecular structure of ovomucoid fixed to a carrier is partly modified, or a stationary phase in which ovomucoid having the molecule partly modified is fixed to a carrier is disclosed. A process for producing an optical isomer separating agent comprises the steps of immobilizing ovomucoid on a carrier and modifying the ovomucoid is disclosed. Furthermore a process for producing an optical isomer separating agent comprises the steps of modifying ovomucoid and immobilizing the modified ovomucoid on a carrier is disclosed.

Method of resolving a mixture of optical isomers

An ovoglycoprotein has a molecular weight of 30,000 daltons which is determined by a matrix-assisted laser desorption ionization time-of-flight type mass spectrometer, an amino acid sequence of 15 residues from the N-terminal represented by Thr-Glu-Ser-Pro-Xaa-Ser-Ala-Pro-Leu-Val-Pro-Ala-Asp-Met-Asp (wherein Xaa represents a cysteine residue or an amino acid residue having a sugar chain linked thereto) (SEQ ID NO:1) and a sugar content of about 25% by weight and free of trypsin-inhibitory activity and a resolution agent for chromatography comprises a fixed phase which comprises a carrier and the foregoing ovoglycoprotein linked to the carrier. The agent for resolving optical isomers is not expensive, and is stable and highly resistant to deterioration by organic solvents, excellent in liquid-flow through properties, has a high sample-loading rate and permits efficient resolution of optical isomers of a chiral compound.

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Kyoto, Japan

Hiroya Fujima