Other

Hiroshi Yamazaki

Burton W Blais

Kouchi Matsumoto

Sushama Joshi

Cameron S Boyd

A stabilized peroxidase conjugate is provided herein. Such conjugate includes a stabilizing quantity of polyethylene oxide or polyvinyl alcohol therein.

Stabilization of peroxidase conjugates in solution

An improved device is provided which may be used, e.g. for immunoassay of lipopolysaccharides or for removing LPS pyrogens from aqueous solutions, or for removing LPS endotoxins from wounds. Such device comprises, in combination, a substrate, e.g. plastic, i.e. polystyrene, polycarbonate, polymethylmethacrylate or polyvinyl chloride, or a woven cloth, i.e. a rayon/polyester cloth or a polyester cloth, or a non-woven cloth, i.e. a rayon/polyester cloth, or a polyester cloth, or paper, which is adapted to receive a sample to be tested, and an oligopeptide, or a hydrophobic polypeptide or a polymyxin, e.g. polymyxin B, polymyxin B.sub.1, polymyxin B.sub.2, polymyxin D.sub.1, polymyxin D.sub.2, or polymyxin E, adhered to the substrate.

Method for using polymyxin-coated substrate for lipopolysaccharide

An improved repetitive, batch-type fermentation process is provided. It includes a first, non-repetitive step of forming a support bearing a film of live and reproductive microorganisms immobilized thereon, e.g. using a cloth support. Then, the process involves the steps of freely suspending and stirring small segments of support cloth bearing a fixed film of live and reproductive microorganisms immobilized thereon within a fermenter containing suitable nutrient liquor to carry out a fermentation process, whereby the microorganisms produce a fermented liquor; withdrawing fermented liquor from the fermenter while retaining the support cloth within the fermenter; adding fresh nutrient to the fermenter containing the support cloth bearing the fixed film of live and reproductive microorganisms immobilized thereon; and repeating the above steps a plurality of times. This provides an improved batch-type fermentation process which minimizes the problems of start-up and which can be readily scaled-up and automated.

Microorganism immobilization

Novel, highly useful, sweet fructose-containing syrups also containing fructooligosaccharides are provided herein by the partial or substantially complete hydrolysis of inulin. The process includes first providing an aqueous solution containing inulin from Jerusalem artichoke tubers or chicory roots. Then the warm aqueous solution of inulin is passed through a column containing a strong acid cation-exchange resin, thereby providing an effluent having a pH of about 2.0-about 3.0. The effluent is then hydrolyzed by heating at a temperature of about 70.degree.-about 100.degree. C., and the hydrolyzate is passed through a column containing of about 6.5-about 7.0. resin, thereby providing an effluent having a pH about 6.5-about 7.0. Optionally, after the hydrolysis step, the hydrolyzate is decolorized by contact with activated or granular charcoal. The effluent is then concentrated to a syrup containing less water than the effluent, e.g. one containing about 40-about 70% solids. The sweet fructose syrup containing oligofructans can be used as truly 'health' sweetener, particularly ideal for elderly people and diabetics. The pulp obtained after the juice extraction is rich in protein and can be used as feed.

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Nepean, Canada

Hiroshi Yamazaki