Hokkaido University

Nitto Electric Industrial Co., Ltd.

Fuso Pharmaceutical Industries, Ltd.

Matsushita Electric Industrial Co., Ltd.

The Kansai Electric Power Co., Inc.

Mitsubishi Denki Kabushiki Kaisha

Nitto-denko Corporation

Hirakawa Iron Works, Ltd.

Hiroshi Tahara

Keiichi Yamamoto

Yusuke Suzuki

Yasuhiko Suzuki

Takeshi Kakuhashi

Yoshihisa Mori

Yuzuru Kitahara

Masayuki Miyazaki

Shinji Nakamura

Toshiaki Takenaka

Tatsunori Okada

Takashi Moriyama

Tetsuro Shimamura

Yoshikazu Tsuru

Toshikazu Kondo

Toshihide Tanaka

Yuichiro Sugita

Jitsuji Otsuki

Enju Nishiyama

Mitsunori Maeda

Yasuhiko Suesada

Junichi Sugioka

Sanyu Narita

Hitoshi Ohta

Seiju Kobayashi

Junji Ohishi

Sinji Itamochi

The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.

Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof

The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.

Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof

As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.

Protein substance having triple helix structure and manufacturing method therefor

A circuit board with high quality is provided by preventing the falling of paste which comes around to the squeegee complementary angle side during the pattern printing or paste filling by the squeegeeing method. While lowering, the squeegee is passed through the inclined portion of a paste removing section ( ) provided on a mask ( ) before pattern printing or paste filling, whereby the paste on the non-printing side (complementary angle) of the squeegee can be removed; thus, a circuit board superior in quality is obtained.

Printing plate, and printing method using the same

The invention is a crystal oscillator having a vibrating plate comprising a vibrating part surrounded by a rim part spaced from the vibrating part except for a narrow connecting part that connects the vibrating part to the rim part in a cantilever fashion. The thickness of the vibrating part and the connecting part are less than the thickness of the rim part. The vibrating part is sandwiched between a pair of casings. Each of the casings has a recess facing the vibrating part, which prevent the vibrating part from contacting the casings. Each surface facing a recess has an electrode positioned thereon and an electrode lead extending from the surface electrode to the connecting part. Each casing has a through-hole positioned to face an electrical lead. A cup-shaped metal lead is placed in each through-hole with the bottom of the cup contacting the electrical lead facing the through-hole. Current to the surface electrodes is supplied through the metal leads.

Crystal oscillator

A printed circuit board having juxtaposed thereon in parallel plural conductive paths, at least a part of which has a different length, extending from one end side to the other end side, wherein the electric resistances of the conductive paths are made substantially same by changing the width of at least a part of the conductive paths.

Printed circuit board having transmission lines with varying thicknesses

A internal reforming type molten carbonate fuel cell is provided in which the process or flow direction of the reforming reaction of a raw fuel gas in the reforming reaction section is countercurrent to the flow direction of the oxidant gas in the cell reaction section. Therefore, a temperature difference in the fuel cell can be suppressed.

Internal reforming type molten carbonate fuel cell

A watertube boiler for all types of boilers including natural circulation, forced circulation and one-through types. The boiler includes a plurality of heat absorption water tubes forming a heat absorption watertube unit in the furnace. The heat absorption watertube unit comprises a relatively dense arrangement of heat absorption water tubes disposed in the combustion path of the burner, such that the flame of the burner impinges on the unit. The heat absorption watertube unit can be arranged in the first stage of a watertube boiler having at least one additional furnace stage receiving exhaust gas from the first stage. The additional stage can be disposed either perpendicularly and horizontally, and the air/fuel mixture in each stage is maintained at a specificied ratio to optimize combustion of the fuel. A method of operating a watertube boiler having three stages is also provided. By using the watertube boiler and method, the generation of nitrogen oxides can be controlled and the volume of the boiler can be reduced.

Watertube boiler and its method of combustion

A resistor sheet input tablet comprising two rectangular resistor sheets each provided at two opposite edges thereof with electrodes, the rectangular resistor sheets being superposed such that the electrodes on one of the resistor sheets lie perpendicularly to those on the other resistor sheets, wherein the resistor sheets have a two resistor layer construction comprising (a) a main resistor layer consisting of a thin metal film deposited thereon which in turn is bonded to (b) an electrically insulating layer and (c) a protective resistor layer formed on the surface of the main resistor layer.

Resistor sheet input tablet for the input of two-dimensional patterns

A part for a slide variable resistor provided on an insulated support thereof with a film resistive layer adapted for a slide terminal to be slid along the surface thereof, which part is characterized in that said film resistive layer comprises a main resistive layer pattern bound to the insulated support and a protective resistive layer pattern coated on said main resistive layer pattern, wherein the protective resistive layer pattern has a larger sheet resistivity than said main resistive layer pattern.

Part for a slide variable resistor

Growing community of inventors

Osaka, Japan

Hiroshi Tahara