Fuji Photo Film Company, Limited

Other

Fujifilm Corporation

Fuji Photo Film C., Ltd.

Hiroshi Shinoki

Osamu Seshimoto

Masashi Ogawa

Yoshikazu Amano

Yukio Sudo

Yoshihide Iwaki

Koki Nakamura

Tadahisa Sato

Yoshihiko Makino

Masayoshi Kojima

Toshikage Hiraoka

Hiroko Inomata

Mitsunori Ono

Junji Nishigaki

Yoshihiro Ashihara

Kouki Nakamura

Tetsuji Tanimoto

Kikuo Hirai

Tadashi Ninomiya

Naofumi Hora

The present invention provides a method for detecting fluorescence by using a solid support to which a probe molecule to be detected is fixed, wherein background is reduced by using a quenching agent. By using present invention, detection sensitivity of a DNA chip can be increased and stable data can be obtained.

Method of reducing background in fluoresence detection

An object of the present invention is to provide a means for simply performing a gene analysis without performing complicated operations. The present invention provides a method for the detection of a nucleic acid, comprising the steps of: performing a PCR reaction or a reverse transcription reaction by adding a template nucleic acid to a solid support on which a nucleic acid is fixed; and hybridizing the nucleic acid fixed on said solid support with the nucleic acid synthesized by the PCR reaction or the reverse transcription reaction.

Method for the detection of gene with DNA micro-array

According to the present invention, there is provided a fluorescent nucleotide represented by the formula: A-B-C, wherein A represents a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue; B represents a divalent linking group or a single bond; and C represents a monovalent group derived from a fluorescent dye having 0 or 1 sulfonic acid group or phosphoric acid group in a molecule. The present invention provides useful fluorescent nucleotides for labeling nucleic acids, specifically, fluorescent nucleotides of which uptake ratio is high in synthetic reaction of nucleic acids.

Fluorescent nucleotides

An agglutination assay method for quantitatively determination of an analyte in a liquid sample using particles bearing an anti-analyte. A non-fluid substance which retains the particles while suppressing the diffusion of the particles therein is used as a medium which is to be a place where the agglutination of the particles takes place. Upon analysis, a solation agent is added to the non-fluid substance medium to increase the fluidity of the non-fluid substance, thereby the particles bearing the anti-analyte can diffuse in the medium to cause the agglutination with the analyte. Preferably, the solation agent is added to the non-fluid medium together with the sample. The non-fluid substance medium containing the particle-labeled anti-analyte can be stored with a higher stability in the dry state. A dry analysis element for enabling such analysis method is also provided.

Agglutination assay method and element in dry system

A process for blocking a device for detection of biochemically active molecules is performed by the steps of:

Blocking of device for detecting biochemically active molecules

An analytical element (typically DNA chip) composed of a solid carrier and a group of nucleotide derivatives or their analogues fixed to the solid carrier can be produced by bringing nucleotide derivatives or the analogues having an alkyne group at one terminal into contact with a zero-valent metal film (e.g., silver metal film or copper metal film) placed on the solid carrier.

DNA chip and its preparation

The present invention provides a measurement chip for a surface plasmon resonance biosensor, which comprises a transparent substrate, a metal membrane located on the transparent substrate and an organic silicon membrane immobilized on the metal membrane and in which the organic silicon membrane is immobilized on the metal membrane via a functional group capable of binding with atoms on the surface of a metal. The measurement chip for a biosensor of the present invention can easily be produced. Using the measurement chip of the present invention, a target substance can be measured with good sensitivity, even if only a small amount of physiologically active substance is immobilized.

Measurement chip for surface plasmon resonance biosensor

A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

Fixation of nucleotide derivatives to solid carrier

Fluorescent substances

An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.

Immunoassay element and process for immunoassay

Agglutination assay method and element in a dry system

An analytical element (typically DNA chip) composed of a solid carrier and a group of nucleotide derivatives or their analogues fixed to the solid carrier via covalent bonding is favorably produced by a method of bringing in a liquid phase a group of nucleotide derivatives or their analogues having a reactive group at one end thereof or its vicinity into contact with a solid carrier having thereon a reactive group in the presence of a transition metal-containing catalyst.

An improved homogeneous enzyme immunoassay process for quantitatively analyzing an antigen by determining the change in the enzymatic activity caused by a reaction between the antigen and an enzyme-labeled antibody. The antigen is reacted with an enzyme-labeled antibody, followed by the reaction with a second antibody capable of recognizing and binding to a different epitope and then with a third antibody capable of recognizing and binding to the second antibody. The enzymatic activity of the labeling enzyme is determined by a water-insoluble substrate. Using the water-insoluble substrate, steric hindrance is enhanced. A highly-sensitive analysis can be carried out by a simple operation even when the antigen has a molecular weight falling within an intermediate range, for example, a range of M.W. 10,000 to 70,000.

Homogenous enzyme immunoassay process

A hemoglobin derivative-containing sample is treated with a treating reagent containing 2-butanol and then immunologically analyzed to determine the quantity of the hemoglobin derivative. By the treatment with 2-butanol, the immunological determination for the hemoglobin derivative, particularly HbA.sub.1c, can be attained with high sensitivity by a simple procedure. Since 2-butanol-containing treating reagent does not affect the enzymatic activity, the homogeneous enzyme immunoassay with high sensitivity is realized.

Method for immunological determination of hemoglobin derivative and

An analysis element for analyzing both amounts of glycated hemoglobin and total hemoglobin in an aqueous liquid sample to determine glycated hemoglobin content ratio in the sample. The element comprises a substrate layer for receiving a reaction mixture after the completion of an immunological reaction between the glycated hemoglobin in the sample and an enzyme-labelled antibody against the glycated hemoglobin, and a reagent layer. The substrate layer contains a non-diffusible substrate which forms a diffusible material in the presence of the enzyme of the enzyme-labelled antibody, the activity of the enzyme being effected relative to the steric hindrance due to the immunological reaction. The reagent layer contains a reagent composition for reacting with the diffusible material to form a dye detectable colorimetrically in a wavelength range which is not effected by an absorption spectrum of the hemoglobin. The total hemoglobin retained in the substrate layer and the dye formed in the reagent layer are colorimetrically analyzed separately, and the glycated hemoglobin content ratio is calculated from respective values. An analysis method using this analysis element is also provided.

Growing community of inventors

Saitama, Japan

Hiroshi Shinoki