Osaka University

The New Industrial Research Organization

Hiroshi Nojima

Takahiro Tougan

Yasuyuki Kita

Daisuke Okuzaki

A method for amplifying a trace amount of mRNA includes adding a dummy RNA to a solution containing the trace amount of mRNA, so as to prepare a mixed solution; synthesizing an anti-sense DNA by reverse transcription, which uses the mixed solution as a template; synthesizing a sense DNA which is complementary to the anti-sense DNA thus synthesized, so as to generate a double-strand DNA made of the sense DNA and the anti-sense DNA; ligating an RNA polymerase promoter sequence to the double-strand DNA thus generated, on a sense DNA 5' end side of the double-strand DNA, so as to prepare a double-strand DNA for amplification; and amplifying, by using RNA polymerase, an RNA from the double-strand DNA for amplification.

Trace mRNA amplification method and use thereof

The present invention relates to multimeric oleamide derivatives having connexin 26 inhibitory activities and embraces the dimer oleamide derivatives represented by the following formula (1) or a pharmacologically acceptable salt thereof: where n denotes an integer of 3, 5, or 8. The novel oleamide derivatives of the present invention can be used not only as research reagents, but also in a wide industrial field because they exhibit useful bioactivities such as cancer metastasis/growth inhibition. Thus they have various applications, such as in medicines, supplements, and functional foods, in addition to cancer-preventive and cancer-therapeutic drugs.

Growing community of inventors

Osaka, Japan

Hiroshi Nojima