Kikkoman Corporation

Fuji-davison Chemical Ltd.

The Japanese Research and Development Association for Bioreactor System

Japanese Research and Development Association for Improvement of Enzyme

Hiroshi Motai

Yaichi Fukushima

Eiichi Nakano

Seiji Murakami

Hiroki Tatsumi

Katsumichi Osaki

Shigetoshi Sugio

Kazutaka Imai

Yoshihiro Ogawa

Haruhide Kawabe

Hirofumi Arimura

Atsushi Masaki

Takashi Ishiyama

Katutoshi Okamura

Kohji Murakami

Tetsuro Fukase

Yutaka Ishida

Harumichi Itoh

Alkaline protease which has leucine or isoleucine in the place of valine at the amino acid number 40 of wild type alkaline protease, a gene encoding the amino acid sequence of alkaline protease which has the substitution as described above, a recombinant DNA comprising said gene, a method of producing the above described alkaline protease, and a DNA fragment used for the expression of a gene.

Alkaline protease, alkaline protease gene, recombinant DNA, DNA fragment

A process for producing protease by cultivating a protease-producing mold in a liquid medium, which is characterized by continuously adding a liquid medium containing a protein material to the culture medium after the substantial termination of proliferation of the mold cells.

Process for producing protease by cultivating a protease-producing mold

A microorganism or enzyme is mixed with an alginate and a silica sol in the presence of water to obtain a liquid mixture having a pH of 3 to 10 and containing an alginate concentration of 0.5 to 3.5% (w/v) and a silica concentration originating from the silica sol of 0.5 to 35% (w/v). The mixture is contacted with a gelling agent in the form of an aqueous solution to obtain a gel containing the microorganism or enzyme.

Microorganism or enzyme immobilization with a mixture of alginate and

A process for producing a seasoning is disclosed wherein a hydrolyzate of soy sauce koji prepared from soy sauce raw materials, in a liquid state at a pH of 2.5 to 8.0, is contacted with an immobilized peptidase and/or immobilized glutaminase in the presence of sodium chloride to react therewith.

Process for producing seasoning

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Noda, Japan

Hiroshi Motai