Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Bio Co., Ltd.

Hiroshi Mizoguchi

Akio Ozaki

Shin-ichi Hashimoto

Yoshiyuki Yonetani

Hirofumi Endo

Satoshi Nakagawa

Masato Ikeda

Mikiro Hayashi

Keiko Ochiai

Satoshi Mitsuhashi

Haruhiko Yokoi

Junko Ohnishi

Seiko Ando

Naoko Tateishi

Akihiro Senoh

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,

Process for producing HMG-CoA reductase inhibitor

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): 

DNA encoding hydroxylase

The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.

Mutant isopropylmalate isomerase

Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.

[object Object]

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,or a ring-closed lactone form thereof;a DNA encoding the protein; and a recombinant DNA comprising the DNA.

Growing community of inventors

Machida, Japan

Hiroshi Mizoguchi