Ajinomoto Co., Ltd.

Hiroshi Miyano

Kazutaka Shimbo

Masakazu Nakazawa

Kazuo Hirayama

Akihisa Yahashi

Shigeru Yamanaka

Katsunori Kobayashi

Kohki Ishikawa

Takashi Ohnuki

Ryosuke Fudou

Tomomi Kuwahara

The present invention provides a method for the analysis of a compound with amino group (e.g., an amino acid or peptide) contained in a sample and convenient manner with a high sensitivity. The compound with amino group in a sample containing the compound with amino group is labeled with a specific carbamate compound such as p-trimethylammonium anilyl-N-hydroxysuccinimidyl carbamate iodide to enhance the selectivity and sensitivity. The present invention is preferably used in conjunction with mass spectrometry such as MS/MS method to facilitate quantitative analysis. The present invention further provides labeling reagents for mass spectrometry.

Method for analysis of compounds with amino group and analytical reagent therefor

The present invention provides a method and apparatus for analyzing compounds with amino group(s) contained in biological organisms. In this method, a compound with amino group(s) is derivatized and then the derivative of the compound with amino group(s) is eluted by liquid chromatography using a stepwise elution means on a concentration gradient. Subsequently, the derivative of the compound with amino group(s) eluted from the liquid chromatography is detected by mass spectrometry.

Method and apparatus for analyzing compounds with amino group

The spore germination inhibitor of the invention is 6-carbamoyl-2-pyridine carboxylic acid which is produced by a microorganism of genus Bacillus, such as and can inhibit germination of sporogenous bacteria resulting in the prevention of spoilage and putrefaction of foods, particularly in combination with D-alanine.

Spore germination inhibitor and food containing the same

Growing community of inventors

Kawasaki, Japan

Hiroshi Miyano