Mitsubishi Kasei Corporation

Mitsubishi Chemical Industries Limited

Wakamoto Pharmaceutical Co., Ltd.

Mitsubushi Kasei Corporation

Hiroshi Kusano

Eiji Miyata

Akihiro Shimura

Hideaki Kiniwa

Masahiko Annaka

Tsuyoshi Ito

Takaharu Itagaki

Hirohisa Kubota

Takayuki Tashiro

Akira Kamata

Takao Uchiyama

Kenji Yokota

Hiroaki Takayanagi

Ryuichi Sugimoto

Toyokazu Sugawara

Tomohiko Kitsukawa

A composite separating agent which comprises a hydrophilic polymer separating agent showing a degree of swelling in water of 10 to 100 ml/g-dry and having a giant network structure incorporated within pores of an organic polymer substrate showing a degree of swelling in water of 20 ml/g-dry or below and a degree of crosslinking of 4% to 100% by mol and having a porous structure as well as a process for producing the same are disclosed. When packed within a column, this composite separating agent having a high mechanical strength is hardly compacted and exhibits an excellent separation performance. Thus it is highly useful in chromatographic separation of biopolymers including proteins.

Composite separating agent

Disclosed herein is an immobilized enzyme inulin-D-fructotransferase carried in a specific anionic exchange resin with pores having a mode radius in the range of 75 to 2,000 angstroms. The immobilized enzyme of the present invention is effective in the production of DFA III from inulin.

Immobilized FTF enzymes

Improved cell culture microcarriers are disclosed herein. These improved microcarriers consist of water-insoluble polymer particles constituted by (meth)acrylic ester, characterized in that the particles have an average diameter of 110-1,000 .mu.m and a density of 1.00-1.20 g/ml in a culture medium as well as positively chargeable chemical moieties distributed at least thereon, the moieties being introduced into the particles by the reaction between the ester and ammonia or amine having 5 or less carbon atoms.

Cell culture microcarriers

A process for producing crosslinked polymer particles which comprises a first step of polymerizing a polymerizable monomer containing from 0.1 to 2.0% by weight, based on the entire polymerizable monomer, of a crosslinkable monomer in a medium in which the polymerizable monomer is soluble but the resulting polymer is insoluble, to obtain crosslinked polymer particles having a particle size of from 0.8 to 10 .mu.m, and a second step of letting the crosslinked polymer particles absorb from 1 to 50 times by weight of a polymerizable monomer containing from 2 to 100% by weight, based on the entire polymerizable monomer, of a crosslinkable monomer in an aqueous medium, followed by polymerization to obtain crosslinked polymer particles having a particle size of from 1 to 30 .mu.m and a crosslinking degree of from 1.0 to 98%.

Process for producing crosslinked polymer particles

A process for producing crosslinked polymer particles which comprises a first step of polymerizing a polymerizable monomer containing from 0.1 to 2.0% by weight, based on the entire polymerizable monomer, of a crosslinkable monomer in a medium in which the polymerizable monomer is soluble but the resulting polymer is insoluble, to obtain crosslinked polymer particles having a particle size of from 0.8 to 10 .mu.m, and a second step of letting the crosslinked polymer particles absorb from 1 to 50 times by weight of a polymerizable monomer containing from 2 to 100% by weight, based on the entire polymerizable monomer, of a crosslinkable monomer in an aqueous medium, followed by polymerization to obtain crosslinked polymer particles having a particle size of from 1 to 30 .mu.m and a crosslinking degree of from 1.0 to 98%

Porous cross-linked polyvinyl alcohol particles which is used for a chromatographic medium is prepared by:

Porous cross-linked polyvinyl alcohol particles, process for producing

A separation medium useful as an adsorbent of liquid chromatography for optical resolution of racemic mixtures is disclosed, which comprises a reversed phase support coated with an optically active amino acid derivative represented by formula (I): ##STR1## wherein R.sub.1 represents a straight or branched alkyl group having from 1 to 3 carbon atoms; and R.sub.2 and R.sub.3 each represents a straight or branched alkyl group having from 4 to 12 carbon atoms which may contain an aromatic group or an unsaturated alkyl group. The separation medium is applicable to optical resolution of a wide range of DL-amino acids and exhibits excellent durability.

Separation medium

A method for producing a separating agent having a fluorine-containing group and a hydrophilic group, wherein a cross-linking component consisting of alkylene di(meth)acrylate and/or glycerine poly(meth)acrylate, and a principal chain component consisting of ester and/or ether having therein a polymerizable vinyl group or isopropenyl group and a functional group are subjected to suspension-polymerization to obtain a cross-linked copolymer, and then the functional group in the copolymer is subjected to chemical modification with a fluorine-containing compound or with both fluorine-containing compound and hydrophilic-group-containing compound. The separating agent of the present invention consists essentially of porous cross-linked copolymer particles having porosity in a range of from 0.5 to 3 ml/g and an average particle size in a range of from 1 to 2,000 .mu.m.

Separating agent with fluoroalkyl group

In a method of separating phenylalanine and cinnamic acid from a mixed solution containing phenylalanine and cinnamic acid, a method of separating phenylalanine and cinnamic acid which comprises subjecting said mixed solution to chromatographic development using a synthetic adsorbent, such as Diaion, Amberlite etc., in the presence of a salt, such as ammonium halides, ammonium sulfate, alkali metal nitrates, ammonium and alkali metal salts of fatty acids, at a concentration in the range where the salting-out effect on cinnamic acid is greater than that on phenylalanine and also both phenylalanine and cinnamic acid can be dissolved, for example, in the range of 0.2-5N.

Method separating phenylalanine and cinnamic acid

A process for the purification of riboflavine-5'-monophosphate comprising subjecting crude riboflavine-5'-monophosphate to chromatography using a column packed with a weakly basic anion-exchange resin having a skeleton of a crosslinked polyacrylic acid ester or polymethacrylic acid ester in which at least a part of the ester linkages thereof are converted into amide linkages represented by the following formula (1): ##STR1## to obtain a fraction of riboflavine-5'-monophosphate containing a reduced amount of impurities from an eluate of the column is disclosed.

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Yokohama, Japan

Hiroshi Kusano