Suntory Holdings Limited

Suntory Limited

Nippon Suisan Kaisha, Ltd.

Hiroshi Kawashima

Sakayu Shimizu

Hideaki Yamada

Harukazu Fukami

Kengo Akimoto

Misa Ochiai

Tomohito Hamazaki

Motoo Sumida

Norifumi Tateishi

Shigeki Sawazaki

Yoshiko Ono

Masashi Matsui

Satomichi Yoshimura

Kenji Katano

Akiko Shiraishi

Kenichi Higashiyama

Yoshiyuki Ishikura

Eiji Sakuradani

Norio Nakamura

Katsuya Nagai

Hiroshige Okaichi

Youko Okaichi

Shiro Watanabe

A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium.

Methods for producing polyunsaturated fatty acid and lipid containing polyunsaturated fatty acid

The object of the present invention is to provide a composition that has preventive or ameliorative action on symptoms or diseases caused by decreased brain function. This composition contains, as its active ingredient, arachidonic acid and/or a compound having arachidonic acid as a constituent fatty acid and, particularly, an alcohol ester of arachidonic acid or a triglyceride, phospholipid or glycolipid in which all or a portion of the constituent fatty acids are arachidonic acid.

Composition having effects of preventing or ameliorating conditions or diseases caused by brain hypofunction

The present invention provides a composition, such as a food and pharmaceutical agent, which comprises dihomo-&#x3b3;-linolenic acid, and which has the effect of preventing or treating skin diseases; a composition such as a food and pharmaceutical agent which comprises dihomo-&#x3b3;-linolenic acid and which has the effect of preventing or treating skin diseases; and a composition which comprises dihomo-&#x3b3;-linolenic acid and which has the effect of preventing or treating diseases related to increased mast cell count.

Composition containing dihomo-&#x3b3;-linolenic acid (DGLA) as the active ingredient

There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid particles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

Microorganisms that extracellularly secrete lipids and methods of producing lipid and lipid particles encapsulating lipids using said microorganisms

A composition having a normalizing action for infradian rhythm and/or a synchronization promoting action for circadian rhythm (biological clock) containing arachidonic acid and/or an arachidonic acid-containing compound.

Composition for normalization of infradian rhythm

Microorganisms that extracellularly secrete lipid particles encapsulating lipids

Composition comprising dihomo-&#x3b3;-linolenic acid (DGLA) as active ingredient

To provide a preventive or ameliorating agent for liver diseases associated with hepatopathy comprising an omega-9 unsaturated fatty acid as an active ingredient.

Preventive or ameliorating agent for liver disease involving hepatopathy

There are provided microorganisms, for example,, having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

Microorganisms that extracellularly secrete lipids and methods of producing lipid and lipid vesicles encapsulating lipids using said microorganisms

The present invention provides a method of efficiently and stably producing LCPUFA-PL. Specifically, the invention provides a method for producing phospholipids that contain LCPUFA as a constituent (LCPUFA-PL), wherein lipid producing cells producing lipids that contain long-chain polyunsaturated fatty acids (LCPUFA) are used as a starting material, the method including a PL extracting step of extracting phospholipids (PL) from defatted cells obtained by extracting triglyceride (TG)-containing oil or fat from the lipid producing cells.

Producing method of phospholipids including long-chain polyunsaturated fatty acids as constituents, and use of such phospholipids

Phospholipids including long-chain polyunsaturated fatty acids as constituents, and use of such phospholipids

A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (=d) to the inner diameter of the culture tank (=D) is d/D=0.30 to 0.6.

Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil

There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

Microorganism secreting out lipid and process for producing the lipid and lipid balls having the lipid encapsulating therein by using the mircroorganism

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Takatsuki, Japan

Hiroshi Kawashima