Toyo Boseki Kabushiki Kaisha

Toyobo Co., Ltd.

Hiroshi Kawaminami

Yoshiaki Nishiya

Takahide Kishimoto

Hiroshi Aiba

Masao Kitabayashi

Yuu Utashima

Shusaku Yanagidani

Yosuke Sumida

Rie Hirao

Yuji Tsuji

Keizo Yoneda

An immunochromatographic test piece is provided containing a control line detection reagent that is not affected by the concentration of an analyte to be detected. The immunochromatographic test piece is for quantifying an analyte in a biological sample, and includes (a) a conjugation pad impregnated with a detection reagent that specifically binds to the analyte, and a control line detection reagent; and (b) a porous membrane pad on which an antibody for capturing the analyte is immobilized upstream, and an antibody for capturing specifically the control line detection reagent is immobilized downstream.

Immunochromatographic test piece

An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.

Glucose dehydrogenase

An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55&#xb0; C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.

Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.

Modified flavin adenine dinucleotide-dependent glucose dehydrogenase

The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sample.

Modified flavin adenine dinucleotide dependent glucose dehydrogenase

The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55&#xb0; C. for 15 minutes in a liquid form compared with the activity before being treated.

The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.

Method for highly expressing recombinant glucose dehydrogenase derived from filamentous fungi

An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors. A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably anfungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

Growing community of inventors

Tsuruga, Japan

Hiroshi Kawaminami