Ntt Docomo, Inc.

Api Corporation

Nippon Shokubai Co., Ltd.

Mitsubishi Chemical Corporation

Ube Corporation

Unibio Corporation

Hiroshi Kawabata

Satoshi Washio

Ryoma Miyake

Masaaki Yamamoto

Takeshi Natsuno

Yasumasa Dekishima

Kazuhiro Yamada

Nobuyuki Watanabe

Dai Kamiya

Takashi Kondo

Mao Asai

Masayuki Tsuda

Atsuki Tomioka

Tomoko Maeda

Fumiaki Miura

Kuniko Asada

Jun Ogawa

Masaki Nagahama

Jun Takehara

Tomohiro Ando

Hisatoshi Uehara

Makoto Hibi

Takanobu Iura

Naoyuki Watanabe

Toyokazu Yoshida

Yuuki Asuma

Koichi Ishida

Ryouhei Katou

Hiroyuki Tanaka

Tatsuya Suzuki

Makoto Ueda

Kuniki Kino

Takeshi Murakami

Hidehito Takayama

Noboru Sakamoto

Naoki Yamane

Youichi Matsumoto

Hisashi Yoshinaga

Yasunori Hattori

Kyoko Endo

Tsugihiko Hidaka

Yasuyo Saito

Kazuaki Kanno

Ryotaro Hara

Tomoko Sasaki

Risa Watanabe

Masaharu Mizutani

Kosuke Ito

Tomoo Yamaguchi

Jingping Qu

Shihomi Suzuki

Yutaka Takemoto

Kyoji Yoshinaka

Takashi C Kondo

The purpose of the present invention is to provide a modified activin A. The present invention provides activin A comprising a modified proregion.

Modified activin A

An object of the present invention is to provide a method of industrially producing a high-purity L-cyclic amino acid more inexpensively and with a high efficiency, from a cyclic amino acid having a double bond at the 1-position. The present invention provides a method in which an L-cyclic amino acid is produced by allowing a cyclic amino acid having a double bond at the 1-position to react with a specific enzyme having a catalytic ability to reduce a cyclic amino acid having a double bond at the 1-position to produce an L-cyclic amino acid.

Production method for L-cyclic amino acids

The present invention provides a novel hydrolase that can industrially produce optically highly pure (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid with high efficiency at low costs, and a production method using the hydrolase.

Hydrolase and method for producing (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid using same

A method of producing high-purity hydroxy-L-pipecolic acids that includes allowing an L-pipecolic acid hydroxylase, a microorganism or cell having the ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture liquid comprising the enzyme and obtained by culturing the microorganism or cell, to act on L-pipecolic acid as a substrate in the presence of 2-oxoglutaric acid and ferrous ion, wherein the L-pipecolic acid hydroxylase (1) acts on L-pipecolic acid in the presence of 2-oxoglutaric acid and ferrous ion to add a hydroxy group to the carbon atom at positions 3, 4, and/or 5 of L-pipecolic acid; and (2) has a catalytic efficiency (kcat/Km) with L-proline that is equal to or less than 7 times the catalytic efficiency (kcat/Km) with L-pipecolic acid.

Method for producing hydroxy-L-pipecolic acid

An object of the present invention is to provide a novel method capable of producing rosuvastatin calcium and intermediates therefor efficiently, inexpensively and with high purity. The present invention provides a method of efficiently producing rosuvastatin calcium and intermediates therefor having a high purity at an industrial scale, without using an extremely low temperature reaction or a special asymmetric catalyst.

Rosuvastatin calcium and process for producing intermediate thereof

A novel method of producing high-purity hydroxy-L-pipecolic acids in an efficient and inexpensive manner while suppressing the production of hydroxy-L-proline is provided. The method includes allowing an L-pipecolic acid hydroxylase, a microorganism or cell having the ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture liquid comprising the enzyme and obtained by culturing the microorganism or cell, to act on L-pipecolic acid as a substrate in the presence of 2-oxoglutaric acid and ferrous ion, wherein the L-pipecolic acid hydroxylase has the properties:

The present invention provides a pipecolic acid 4-hydroxylase protein exemplified by the following (A), (B), and (C), having activity to react with L-pipecolic acid in the presence of 2-oxoglutaric acid and iron(II) ions to produce trans-4-hydroxy-L-pipecolic acid, and a method for producing 4-hydroxy amino acid, which method comprises reacting the pipecolic acid 4-hydroxylase protein, cells containing the protein, a treated product of the cells, and/or a culture liquid obtained by culturing the cells, with &#x3b1;-amino acid to produce 4-hydroxy amino acid:

Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same

A monomer composition containing an unsaturated polyalkylene glycol ether-based monomer is provided having excellent stability. The monomer composition contains an unsaturated polyalkylene glycol ether-based monomer represented by the following chemical formula (1):YO(RO)R&#x2003;&#x2003;(1)[in the formula, Y represents an alkenyl group having 2 to 7 carbon atoms; RO represents one or two or more types of oxyalkylene groups having 2 to 18 carbon atoms; n represents an average addition, mole number of oxyalkylene groups and is a number of 5 to 500; and Rrepresents a hydrogen atom, or a hydrocarbon group having 1 to 30 carbon atoms], an organic acid, and water, and having a pH of 4 to 13.

Monomer composition containing unsaturated polyalkylene glycol ether-based monomer, method for producing composition thereof, polymer obtained using composition thereof, and method for producing polymer thereof

According to the present invention, it becomes possible to perform a process for converting into an &#x3b1;-substituted cysteine represented by general formula (1) or a salt thereof at low cost and on an industrial scale by employing a process that is routed through a compound represented by general formula (3) to a compound represented by general formula (6). Particularly, by employing a process that is routed through a compound represented by general formula (7-2), it becomes possible to detach a tert-butyl protection group in a simple manner and to produce the compound represented by general formula (1) with high purity. Furthermore, by employing a process that is routed through tert-butylthiomethanol or a process that is routed through a compound represented by general formula (9), it becomes possible to produce a compound represented by general formula (2) without generating bischloromethylether that is an oncogenic substance. In the production of an &#x3b1;-substituted-D-cysteine or a salt thereof, it becomes possible to perform a process for converting the compound represented by general formula (2) into a compound represented by general formula (3S) in one step by allowing an enzyme or the like to act on the compound represented by general formula (2).

Method for producing alpha-substituted cysteine or salt thereof or synthetic intermediate of alpha-substituted cysteine

A monomer composition containing an unsaturated polyalkylene glycol ether-based monomer is provided having excellent stability. The monomer composition contains an unsaturated polyalkylene glycol ether-based monomer represented by the following chemical formula (1):YO(RO)R&#x2003;&#x2003;(1)[in the formula, Y represents an alkenyl group having 2 to 7 carbon atoms; RO represents one or two or more types of oxyalkylene groups having 2 to 18 carbon atoms; n represents an average addition mole number of oxyalkylene groups and is a number of 5 to 500; and Rrepresents a hydrogen atom or a hydrocarbon group having 1 to 30 carbon atoms], an organic acid, and water, and having a pH of 4 to 13.

The present invention aims to provide a method for efficiently producing hydroxy-L-lysine. The present invention provides a method for producing hydroxy-L-lysine, the method comprising allowing 2-oxoglutarate-dependent L-lysine hydroxylase, a cell containing 2-oxoglutarate-dependent L-lysine hydroxylase, a processed product of the cell, and/or a culture broth obtained by culturing the cell, to act on L-lysine to produce hydroxy-L-lysine represented by the following General Formula (I) (wherein each of R, Rand Rrepresents a hydrogen atom or hydroxyl group, with the proviso that at least one of R, Rand Rrepresents a hydroxyl group). 

L-lysine hydroxylase and production method for hydroxy-L-lysine and hydroxy-L-pipecolic acid using same

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Tokyo, Japan

Hiroshi Kawabata