Ajinomoto Co., Ltd.

Hiroshi Izui

Yoshihiko Hara

Hisao Ito

Kazuhiko Matsui

Mika Moriya

Hisashi Yasueda

Eiji Ono

Tsuyoshi Nakamatsu

Yury Ivanovich Kozlov

Konstantin Vyacheslavovich Rybak

Ekaterina Aleksandrovna Slivinskaya

Akito Chinen

Joanna Yosifovna Katashkina

Jun Nakamura

Seiko Hirano

Takahiro Asano

Yasuyuki Watanabe

Yoshihiro Usuda

Osamu Kurahashi

Nobuharu Tsujimoto

Hiroki Kawashima

Masakazu Sato

Hiromi Noguchi

Naoki Akiyoshi

Ranko Nishi

Seizaburo Shiraga

Noriko Murayama

Hiromi Ohtaki

Kayo Moriguchi

The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Use of phosphoketolase for producing useful metabolites

The present invention describes an L-glutamic acid-producing bacterium which belongs to the genusor, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

L-glutamic acid producing bacterium and a method for producing L-glutamic acid

L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genusorand having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

L-glutamic acid-producing bacterium and method for producing L-glutamic acid

A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase &#x3b1;-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.

Microorganism producing an amino acid of the L-glutamic acid family and a method for producing the amino acid

A microorganism which has an L-amino acid producing ability and has been modified so that succinate dehydrogenase activity and &#x3b1;-ketoglutarate dehydrogenase activity are decreased is cultured in a medium to produce and accumulate an L-amino acid in the medium or cells of the microorganism, and the L-amino acid is collected from the medium or cells to produce the L-amino acid.

Method for producing an L-amino acid

A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Method for producing L-glutamic acid by fermentation accompanied by precipitation

L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

L-glutamic acid-producing microorganism and a method for producing L-glutamic acid

A gene isolated from a microorganism selected from the group consisting ofbacteria,bacteria, andbacteria, and encoding a Rep protein or a homologue thereof is described. A plasmid containing the gene which is autonomously replicable in Enterobacteriaceae bacteria is also described. A Enterobacteriaceae microorganism containing the plasmid is also described.

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In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.

Method for producing L-amino acid

L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting the otsA gene derived from a coryneform bacterium source, coding for trehalose-6-phosphate synthase, to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium.

OtsA gene encoding trehalose-6-phosphate synthase from a coryneform bacterium

L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.

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Kawasaki, Japan

Hiroshi Izui