Nippon Telegraph and Telephone Corporation

Hiromichi Mizutani, Deceased

Koichi Fujiwara

Koichi Arishima

Shuichi Shibata

Yasushi Hoshino

Mitsutoshi Hoshino

Hiroko Mizutani

Juichi Noda

Hiroko Misutani

A device for collecting a specimen, including (a) a test vessel for containing a solution containing an immunocomplex, which is a complex as a result of an antigen-antibody reaction between a specimen and a magnetic-labeled antibody containing a magnetic micro-particle and an antibody fixed to the micro-particle, (b) an external magnetic field generating device for generating a magnetic field, preferably a gradient magnetic field and applying it to the solution in the test vessel to effect local concentration of the immunocomplex to a predetermined position, (c) a magnetic member for collecting the immunocomplex at the position of local concentration, and (d) a moving mechanism for achieving relative movement between the magnetic member and the test vessel.

Device for collecting or preparing specimens using magnetic

A method for collecting a specimen comprising (1) subjecting a specimen and a magnetic-labeled antibody comprising a magnetic micro-particle and an antibody fixed to the micro-particle to an immunoreaction to form a reacted immunocomplex, (2) concentrating the reacted immunocomplex locally at a selected region by applying an external magnetic field, preferably a gradient magnetic field, which is produced by an electromagnet and a permanent magnetic piece so as to concentrate the magnetic flux at the selected region, and (3) collecting the immunocomplex by direct magnetic adsorption toward the magnetic pole at the region of local concentration.

Method for collecting and preparing specimens for immune reactions

A laser magnetic immunoassay (LMIA) technique is presented which combines the high detection sensitivity of the radioimmunoassay (RIA) technique with the simplicity of particle agglutination (PA) technique. The LMIA technique dispenses with the separation step of bound and free species (B/F separation), yet provides detectivity of the order of picogram of viral species per milliliter of analyte solution. The procedure includes of the steps of: preparing a superparamagnetic-labeled body of antigen or antibody; subjecting a specimen sample and the magnetic-labeled body to a specific immunoreaction to produce a reacted body; subjecting the reacted body containing both bound and free species to a spot magnetic field gradient; irradiating the spot with a laser beam; measuring the intensity of the outgoing light from the spot; and making a quantitative determination of the virus according to the time difference in the outgoing light signals generated by bound and free species.

Laser magnetic immunoassay method and apparatus therefor and

A laser magnetic immunoassay (LMIA) method which enables detection of less than picograms of target analyte in milliliter of analyte solution. The method involves the steps of: affixing an antibody or antigen on the surface of non-magnetic carrier particles; immunoreacting the affixed antibody or antigen to capture a target analyte contained in a specimen; preparing magnetic labeled microparticles treated so as to bind with the target analyte; reacting the labeled complex to sandwich the target analyte between the magnetic particles and the non-magnetic carrier particles; separating the free species from the bound species by centrifugation; dispersing the precipitated solid to make an analyte solution; applying a spot magnetic field gradient on the analyte solution and irradiating a selected spot with a laser beam; and analyzing the resulting interference patterns to quantitatively determine the quantity of target analyte.

Laser magnetic immunoassay method and apparatus thereof

An in-situ laser magnetic immunoassay ('LMIA') method which eliminates the step of B/F separation generally required in the labeling method of immunoassays. The laser magnetic immunoassay permits a quantitative determination of a target immunological substance, for example, an antigen, an antibody, lymphocytes, viruses, tumorous cells and infections cells, in an analyte solution containing both bound and free species. A transitory increase in the magnetophoretic scattering of laser beam is observed when the analyte solution contains magnetic-labeled, bound target analyte, while no such increase is observed in a control test solution, containing only the relevant reagents. A magnetophoretic LMIA apparatus is provided which includes a magnetic gradient generating device which forms an integral part of the in-situ LMIA.

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Tokyo, Japan

Hiromichi Mizutani, Deceased