National Center of Neurology and Psychiatry

Takara Bio Inc.

Nippon Medical School Foundation

Hiromi Kinoh

Takashi Okada

Shin'ichi Takeda

Tatsuji Enoki

Junichi Mineno

Toshikazu Nishie

Hironori Okada

An object of the present invention is to develop and provide a method for conveniently introducing a nucleic acid, a peptide, and/or a low-molecular-weight compound into an empty capsid with viral early infection activities kept. The present invention provides a method for producing a drug delivery particle, comprising the steps of: mixing an empty capsid or an empty particle with a drug including a nucleic acid, a peptide, and/or a low-molecular-weight compound in a solution comprising 0.1 to 20% of a surfactant; and keeping the obtained mixed solution at &#x2212;5 to 50&#xb0; C. to introduce the drug into the empty capsid or the empty particle.

Drug delivery particle and method for producing the same

The present invention provides: a mutant of adeno-associated virus (AAV) capsid protein, which contains at least one amino acid substitution in PLA2 domain when compared with the amino acid sequence for wild-type AAV capsid protein; a nucleic acid encoding the mutant; a cell containing the nucleic acid; a method for producing a recombinant AAV particle, comprising a step of culturing the cell to produce the recombinant AAV particle; a recombinant AAV particle containing the mutant; a composition containing the recombinant AAV particle; and a method for transferring a gene into a target cell, comprising a step of bringing the recombinant AAV particle into contact with the target cell.

Mutant of adeno-associated virus (AAV) capsid protein

Growing community of inventors

Tokyo, Japan

Hiromi Kinoh