Aisin Seiki Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Hiroko Uchiyama

Naoto Kagiyama

Masayoshi Momiyama

Satoshi Fujita

Osamu Asami

Yukio Yamada

Yasumitsu Kondoh

Takekazu Yamamoto

Hidehiko Sugiyama

A method of detecting nucleic acid utilizes hybridization between the nucleic acid to be detected which is present on an immobilizing support and a DNA probe which is complementary to at least a portion of the desired nucleotide sequence of the nucleic acid. The present DNA probe includes a hapten attached via a linker which a hapten is gibberellin or a gibberellin derivative. After hybridization by reaction between the nucleic acid to be detected and the DNA probe, the unreacted DNA probe is removed, and then labelled anti-hapten antibody is allowed to bind with the DNA probe of the hybrid and the labelling is used to detect the nucleic acid. Accordingly, stable detecting sensitivity may be achieved regardless of the label mixing ratio or labelling ratio, and thus regardless of the nucleotide sequence composition of the nucleic acid being tested.

Method of detecting nucleic acid

Growing community of inventors

Nagoya, Japan

Hiroko Uchiyama