Shimadzu Corporation

Hiroko Morinaga

Shigeki Kajihara

Masahiro Ikegami

A data matrix in which pixel numbers are assigned to the vertical direction, m/z values are assigned to the horizontal direction, and intensity values are used as terms is generated from data obtained by peak detection (S), and after the standardization of the data is executed so that the norms of the intensities of the pixel space are set to 1 for each m/z, the peaks (m/z values) are classified into a plurality of clusters by performing clustering in the m/z direction (Sand S). Since the probability of isotope peaks or adduct ion peaks derived from the same substance being consolidated into the same cluster increases, unnecessary peaks can be accurately removed by removing unnecessary isotope ion peaks and the like using m/z differences or intensities in the clusters (Sand S).

Mass spectrometry data analysis method and analysis device

The present invention provides a method and apparatus for efficiently handling a large amount of data collected by an imaging mass analysis to present significant information for the analysis of the tissue structure of a biological sample or other objects in an intuitively understandable form for analysis operators. For each pixelon a sample, a mass-to-charge ratio m/z(i) corresponding to the maximum intensity MI(i) in a mass spectrum is listed, and the largest value MII of the maximum intensities of all the pixels are extracted. A color scale corresponding to the intensity values within a range of 0 to MII is defined. For each pixel, the maximum intensity MI is compared with the color scale to assign a color to that pixel. A mapping image with the pixels shown in the respective colors is created and displayed. Simultaneously, a spectrum showing the relationship between MI(i) and m/z(i) of all the pixels is created in such a manner that the peak colors correspond to the pixel colors on the mapping image. The mapping image shows the tissue structure of the sample. By comparing this image with the spectrum, the m/z of a noticeable substance in the sample can be identified.

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Kyotanabe, Japan

Hiroko Morinaga