Other

University of Tsukuba

Center of Biotechnology of Sfax, University of Sfax, Mesrs

Tsukuba Biosystem, Ltd.

Hiroko Isoda

Takaaki Maekawa

Takashi Koyama

Norio Sugiura

Kazuhiro Nagata

Mikio Kitahara

Syuichi Oka

Shinichi Yokota

Mitsutoshi Nakajima

Marcos Neves

Yuhei Inamori

Ilyes Dammak

Masako Tasaki

Sami Sayadi

The present invention relate to a highly efficient and novel method, using clean technologies, for obtaining a natural bioactive concentrate that is rich on polyphenols from olive mill water (OMW). The clean technologies integrate centrifugation, a drowning-out crystallization-based separation process, and vacuum evaporation. The method provides a highly-concentrated polyphenol isolate (up to 99% (mass fraction)) from other components presents in OMW, with up to half of the polyphenol content being hydroxytyrosol. The isolated polyphenols exhibit anti-oxidant, anti-microbial, anti-inflammatory, and anti-carcinogenic activities; they can be prepared as solid particles, as an aqueous solution, in an emulsion, or as lipidic-based nanoparticles. The isolated polyphenols can be used in the food industry, cosmetic industry, or pharmaceutical industry.

Method for isolating polyphenols from olive mill water

A mushroom fungus belonging to the order of is cultured with good efficiency by using a liquid culture medium in such a way as to prepare the separated from the culture medium such that the culturing liquid can be served for food use. A liquid culture medium containing 3 to 16 g/liter of sucrose and 1 to 6 g/liter of maltose as a carbon source and 0.3 to 1.2 g/liter of yeast extract as a nitrogen source is inoculated with and culturing is conducted by blowing into the culture medium sterilized air of 20 to 90% of the oxygen concentration.

Method for culturing agaricus edible fungus

The present invention provides a bioassay system, for detection of hazardous chemical substances, natural toxic substances in the environment and unknown toxic compounds, with high sensitivity, simplicity and speed. The invention provides cells and a method of using the cells for use in the bioassay system. The cell provided by the invention contains a heat shock factor binding DNA sequence and a transcriptional regulatory sequence necessary on an occasion of stress induction as a transcriptional regulatory factor binding site. The cell also possesses a reporter gene under the control of the promoter. The reporter gene is connected, on the downstream side, to the SV40pA signal without any intervening intron.

High-sensitive detection of environmental pollutants

Growing community of inventors

Tsukuba, Japan

Hiroko Isoda