Fuji Photo Film Company, Limited

Hiroko Inomata

Masayoshi Kojima

Osamu Seshimoto

Junji Nishigaki

Yukio Sudo

Hiroshi Shinoki

Kouki Nakamura

Kazuya Takeuchi

According to the present invention, there is provided a fluorescent nucleotide represented by the formula: A-B-C, wherein A represents a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue; B represents a divalent linking group or a single bond; and C represents a monovalent group derived from a fluorescent dye having 0 or 1 sulfonic acid group or phosphoric acid group in a molecule. The present invention provides useful fluorescent nucleotides for labeling nucleic acids, specifically, fluorescent nucleotides of which uptake ratio is high in synthetic reaction of nucleic acids.

Fluorescent nucleotides

Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X&#x2014;Y&#x2014;Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein Rto Rrepresent an alkyl group; Vto Vrepresent a hydrogen atom or a substituent; Lto Lrepresent a methine group; W represents an oxygen atom, a sulfur atom, &#x2014;C(R)(R)&#x2014; or &#x2014;N(R)&#x2014; wherein Rto Rrepresents an alkyl group; Q represents a nitrogen atom or &#x2014;C(V)&#x2014; wherein Vrepresents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in Ror R 

Fluorescent nucleotides and labeling methods using the same

Fluorescent substances

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Saitama, Japan

Hiroko Inomata