Fujifilm Corporation

Hiroko Inomata

Toshihiro Mori

Nobuhito Masuda

Yoshihiko Makino

Yoshihiko Abe

Shigeki Kageyama

Kazuya Kawasaki

Rie Hando

Rie Iwata

Tomoko Mori

Mariejoy Jumawid

Yumiko Takeshita

An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived fromorsp. and cholesterol oxidase derived fromsp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.

Method for measuring high density lipoprotein cholesterol

It is an object of the present invention to provide a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which is able to selectively measure low density lipoprotein cholesterol in a body fluid without using a low density lipoprotein cholesterol-selective surfactant having a risk of generating endocrine-disrupting chemicals. The present invention provides a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which comprises measuring low density lipoprotein cholesterol (LDL-C) by using (a) cholesterol esterase and (b) cholesterol oxidase or cholesterol dehydrogenase, in the presence of a polyoxyethylene-polyoxypropylene copolymer and a polyglyceryl ether.

Method for measuring low density lipoprotein cholesterol

It is an object of the present invention to reduce influence of hemolysis in a dry analytical element used for measurement of components in a body fluid sample such as blood. The present invention provides a method for producing a dry analytical element for body fluid component measurement comprising at least a reagent layer containing an HOcolor developing reagent and a spreading layer provided on the reagent layer, which comprises steps of providing a spreading layer substrate on the reagent layer containing an HOcolor developing reagent and preparing a spreading layer by coating a low-viscosity solution containing oxidase to the spreading layer substrate and then coating a high-viscosity solution containing other reagent components than oxidase thereto.

Dry analytical element capable of reducing influence of hemolysis for body fluid component measurement

A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pellet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.

Method for separating and purifying RNA

A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 &#x3bc;l or less per 1 cmof the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.

Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid

A functional group-introduced polyamide solid phase comprising: a polyamide solid phase having an amido group; and an isocyanate compound having an isocyanate group and a functional group, wherein the functional group of the isocyanate compound is introduced onto a surface of the polyamide solid phase by reacting the amido group of the polyamide solid phase with the isocyanate group of the isocyanate compound.

Functional group-introduced polyamide solid phase

The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.

Growing community of inventors

Asaka, Japan

Hiroko Inomata