Seikagaku Corporation

Seikagaku Kogyo Kabushiki Kaisha

Hiroko Habuchi

Koji Kimata

Osami Habuchi

Sakaru Suzuki

Masashi Kobayashi

A glycosaminoglycan 6-O-sulfotransferase having an activity of transferring sulfate to a hydroxyl at position 6 of a glycosamine residue of a glycosaminoglycan, which has a ratio of relative activities to substrates satisfying completely desulfated N-acetylated (CDSNAc) heparin/completely desulfated N-resulfated (CDSNS) heparin &#x2267;0.05 and a molecular weight as calculated from constituent amino acids of from 53,000 to 58,000 daltons.

Sulfotransferase and DNA encoding the enzyme

The hexuronic acid derivative represented by the following formula 1 or a salt thereof is used as an active ingredient of a heparin/heparan sulfate sulfotransferase inhibitor.  In the formula, each of R, R, and Rindependently represent(s) SOor H which may have a substituent, provided that at least one thereof represents SO; X represents OR, SR, N(R), or C(R), Rindependently represents H, alkyl, alkenyl, alkynyl, acyl, aryl, or aralkyl group; one of Rand Rrepresents COOH while the other represents H; and the wavy line represents &#x3b1;-glycosidic bond or &#x3b2;-glycosidic bond.

Sulfotransferase inhibitor

A glycosaminoglycan 6-O-sulfotransferase having an activity of transferring sulfate to a hydroxyl at position 6 of a glycosamine residue of a glycosaminoglycan, which has a ratio of relative activities to substrates satisfying completely desulfated N-acetylated (CDSNAc) heparin/completely desulfated N-resulfated (CDSNS) heparin&#x2267;0.05 and a molecular weight as calculated from constituent amino acids of from 53,000 to 58,000 daltons.

Sulfate transferase and DNA encoding this enzyme

An oligosaccharide having an affinity for fibroblast growth factor, which is composed of 8 to 18 monosaccharide residues, wherein a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine and a process for producing the oligosaccharide comprising digesting heparan sulfate.

Oligosaccharide having affinity for fibroblast growth factor and process

The present invention provides a heparan sulfate 6-O-sulfotransferase for selectively introducing sulfate group into hydroxyl group at C-6 of glucosamine of heparin and heparan sulfate, having the following properties: (i) sulfate group is selectively transferred from a sulfate group donor to hydroxyl group at C-6 of N-sulfoglucosamine residue; (ii) sulfate group is transferred to CDSNS-heparan, but sulfate group is not transferred to chondroitin and chondroitin-4-sulfate; (iii) optimum reaction pH is in a range of pH 6-7; (iv) optimum ionic strength is in a range of 0.1-0.3 M (in the case of sodium chloride); and (v) the enzyme activity is inhibited by dithiothreitol and adenosine-3',5'-diphosphate, and activated by protamine.

Heparan sulfate 6-O-sulfotransferase

Heparan sulfate 2-O-sulfotransferase having the following properties is obtained from, for example, CHO cells:

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Nagoya, Japan

Hiroko Habuchi