Morinaga Co. Ltd.

Taiyo Fishery Co., Ltd.

Hiroki Murakami

Yasuhiko Sasamoto

Shuichi Hashizume

Shoji Kimura

Tadakazu Tamai

Nobuyuki Sato

Kazuhiko Kuroda

The present invention provides cell lines capable of indefinite growth which are produced by transforming leukocytes isolated from peripheral blood of fish with a oncogene, a method of establishing the cell line, a method of recovering immunologically active substances from the culture of the cell lines. It has been difficult to maintain continuous cultures of fish leukocytes. The present invention provides cell lines capable of indefinite growth and a method of producing immunologically active substances, e.g., immunologically acitive substances, using these cell lines.

Cell lines produced by transforming leukocytes of fish with oncogenes

A method of assaying immunization stimulating activity comprising (a) immunizing a lymphocyte in vitro by culturing the lymphocyte in contact with an immobilized antigen in a medium containing a test substance and (b) detecting an immunoglobulin, which is produced and secreted into the medium by the immunized lymphocyte and bound to the immobilized antigen, by counting the immunostained spots deposited in situ on the surface of the immobilized antigen.

Method of assaying immunization stimulating activity

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Fukuoka, Japan

Hiroki Murakami