Gekkeikan Sake Company Limited

Toyo Boseki Kabushiki Kaisha

Toyobo Co., Ltd.

Hiroki Ishida

Yoji Hata

Yoshiaki Nishiya

Masao Kitabayashi

Eiji Ichikawa

Satoshi Imayasu

Rie Hirao

Hiromoto Hisada

Koji Suginami

Akitsugu Kawato

Yasuhisa Abe

The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.

Protein having fructosyl valyl histidine oxidase activity, modified protein, and use of the protein or the modified protein

According to the protein expression system in which a variety of desired useful proteins are highly produced by fusing a coding region of useful protein gene to the downstream of a promoter region of a tyrosinase-encoding gene (melO) of by a usual method of DNA manipulation, transferring a plasmid containing the resulting novel fusion gene into and incubating the thus-obtained transformant, various proteins can efficiently be produced at a high purity and in a high yield.

High expression system of proteins

Growing community of inventors

Kyoto, Japan

Hiroki Ishida