Other

National Institute of Advanced Industrial Science and Technology

Astellas Pharma Gmbh

Hiroki Higashibata

Ikuo Matsui

Michihiko Kobayashi

Yoshiteru Hashimoto

Yutaka Kawarabayashi

The present invention provides a vector containing a DNA construct containing a first promoter that is a nitrilase gene promoter, a multicloning site and a nitrilase transcription regulatory protein gene (particularly preferably a structural gene is ligated into a multicloning site and a second promoter is placed at the upstream of the nitrilase transcription regulatory protein gene), and the like. The present invention affords a high expression system capable of expressing a large amount of useful proteins by actinomycete host represented by actinomycete belonging to the genusand the like beneficially used for the production of useful proteins.

Inducible high expression system

The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such asin the usual manner to create a transformant, and culturing the transformant.

DNA helicase

Growing community of inventors

Tsukuba, Japan

Hiroki Higashibata