National Institute of Advanced Industrial Science and Technology

Toppan Printing Co., Ltd.

Hiroki Ashiba

Makoto Fujimaki

Masato Yasuura

Takayuki Shima

Susumu Takahashi

Shu Tanabe

To detect a target substance accurately and effectively, a target substance detection deviceincludes: a liquid-sample introducing plateformed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unitconfigured to be able to irradiate the liquid-sample introducing platewith light from the rear face; a first magnetic field application unitdisposed on the side of the surface of the liquid-sample introducing plate, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate; and an optical-signal detection unitdisposed on the side of the surface of the liquid-sample introducing plate, and enabling detection of a change in optical signal based on the propagated light between before and after application of the magnetic field by the first magnetic field application unit

Target substance detection device and target substance detection method

A target substance detection device detecting a target substance using magnetic particles includes a detection chip, a light irradiation unit, and a magnetic field application unit, wherein the detection chip includes a light transmissive member having a sensing surface arranged on a surface constituting a top surface relative to a bottom surface, an inclined surface, and a main body portion capable of receiving light and guiding the light through the interior, the light transmissive member having a light directing structure in which the light applied from the top surface side and passed through the inclined surface is directed via the main body portion to the sensing surface under the condition of total reflection, the light irradiation unit is operable to irradiate the sensing surface with light under the condition of total reflection via the light directing structure, and the magnetic field application unit.

Target substance detection chip, target substance detection device, and target substance detection method

The invention relates to a method that detects the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc. The invention also relates to an optical detection device wherein a liquid holding part has a sensing plate that can form a near field on a surface with light irradiated from a side of a rear face under a condition of total reflection, the liquid holding part being capable of holding a liquid sample that has a target substance and a magnetic particle that forms a conjugate with the target substance on the surface of the sensing plate, a light irradiation unit, a light detection unit, and a magnetic field application unit configured to apply a magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.

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Ibaraki, Japan

Hiroki Ashiba