Mitsui Chemicals, Inc.

National Institutes of Biomedical Innovation, Health and Nutrition

Savid Therapeutics Inc.

Hiroki Akiba

Tsuneji Suzuki

Shinichi Banba

Yoshiyuki Totani

Kosuke Mano

Haruhiko Kamada

Taisuke Nakayama

Kouhei Tsumoto

Finding a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. Producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.

Method for identifying drug-discovery target protein for development of antibody drug, and method for producing antibody against target protein

Growing community of inventors

Ibaraki, Japan

Hiroki Akiba